Novel Method for Producing Differentiated Cells

A method for producing mature megakaryocytic cells comprising:

• forcibly expressing an oncogene and any one of the following genes, (a) to (c), in hematopoietic progenitor cells, CD34-positive cells, or megakaryocytic progenitor cells without multi-polyploidization and culturing and growing the cells:
• (a) a gene that suppresses the expression of a p16 gene or a p19 gene;
• (b) a gene that suppresses the expression of an Ink4a/Arf gene;
• and
• (c) a polycomb gene;
• and
• differentiating the grown cells into mature megakaryocytic cells.

A cell population comprising megakaryocytic progenitor cells without multi-polyploidization or mature megakaryocytic cells into which an oncogene and any one of the following genes, (a) to (c), are introduced:

• (a) a gene that suppresses the expression of a p16 gene or a p19 gene;
• (b) a gene that suppresses the expression of an Ink4a/Arf gene;
• or
• (c) a polycomb gene.

A kit for producing pre-multinucleation megakaryocytic progenitor cells, mature megakaryocytic cells, or platelets comprising expression vectors for forced expression of an oncogene and any one of the following genes, (a) to (c):

• (a) a gene that suppresses the expression of a p16 gene or a p19 gene;
• (b) a gene that suppresses the expression of an Ink4a/Arf gene;
• and
• (c) a polycomb gene.