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US 2012/0238020 A1Published application
Megakaryocyte and Platelet Production from Stem Cells
AssigneeNew York Blood Center, Inc.
InventorsW. Beau Mitchell, Mauro P. Avanzi
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Abstract
Methods for obtaining purified populations of megakaryocytes and platelets by ex vivo culture of stem cells are provided herein.
Claims13
A method for producing platelets in vitro comprising:
- (1) culturing stem cells in a first growth medium to produce a megakaryocyte progenitor cell population;
- (2) maturing the expanded megakaryocyte progenitor cells in an artificial bone marrow niche environment comprising a second growth medium in the presence of an oxygen concentration between about 10% and about 30% PO2 to differentiate the megakaryocyte progenitor cells into megakaryocytes;
- (3) isolating the mature megakaryocytes;
- (4) culturing the mature megakaryocytes in a three-dimensional matrix and a third growth medium and in the presence of an oxygen concentration between about 10% and about 30% PO2 and a shear stress between about 100 and 400 μl/min to produce platelets;
- and
- (5) collecting the platelets.
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A platelet production system for the ex vivo production of platelets comprising:
- a bioreactor for expansion of stem cells in the presence of a first growth medium in fluid communication with;
- a maturation chamber comprising an artificial bone marrow niche and a second growth medium, wherein the maturation chamber is in fluid communication with;
- a cell separation chamber for selecting mature megakaryocytes which is in fluid communication with;
- a platelet production module comprising a plurality of platelet production chambers, a three-dimensional matrix, a third growth medium, and a plurality of pumps for moving the third growth medium across the platelet production chambers, wherein the platelet production module is in fluid communication with;
- a platelet collection chamber.
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Dependencies are inferred from the claim text.