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US 2012/0237927 A1Published application
Method of Mapping of mRNA Distribution With Atomic Force Microscopy Comprising Dendron
OwnerJoon Won Park, Yu Jin Jung +2inventors
InventorsJoon Won Park, Yu Jin Jung, Yu Shin Park, Hong Gil Nam
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Abstract
The present invention relates to a method of mapping of mRNA distribution, comprising the steps of a preparing a probe DNA attached to a apical liner region of the dendron on AFM cantilever where the probe DNA can specifically hybridize a target mRNA and measuring specific adhesive force between the probe DNA and the target mRNA on sectioned tissue at nanometer resolution.
Claims7
1-5. (canceled)
A method for mapping mRNA distribution on a sectioned tissue sample, said method comprising:
- scanning the sectioned tissue sample with an atomic force microscope (AFM) comprising a probe DNA that is capable of selectively hybridizing to a target mRNA;
- and
- determining the level of target mRNA distribution within the sectioned tissue sample using the adhesive force measured with the atomic force microscope,
- wherein the probe DNA is attached to an AFM cantilever via a dendron comprising:
- a plurality of termini that are attached to the surface of the AFM cantilever;
- and
- a single apical linear region that is attached to the probe DNA.
4 more elements
Dependencies are inferred from the claim text.