Surface coating for biomolecule immobilisation and minimisation of non-specific binding on surfaces for biomedical diagnostics

A coating for a solid substrate, the coating comprising:

• a first siloxane layer;
• and
• a second layer comprising a chemical functionality
• wherein the first and second layers are plasma polymerised onto the substrate, the coating swellable on contact with an aqueous solution by factor of at least 2, based on RMS (root mean square) roughness swelling measurement.

Use of a water swellable coating for a solid substrate the coating comprising:

• a first siloxane layer;
• and
• a second layer comprising a chemical functionality
• wherein the first and second layers are plasma polymerized onto the substrate, the coating swellable on contact with an aqueous solution by factor of at least 2, based on RMS (root mean square) roughness swelling measurement as a tool for biological discovery and/or biomedical detection, in medical imaging and/or therapeutic applications such as cell labeling, targeted drug delivery, targeted gene delivery, biosensing, cell separation, cell purification and imaging.