US 2012/0231969 A1 · PROTEIN ARRAYS AND USES THEREOF

Abstract

Illustrative embodiments herein disclosed relate to protein arrays, methods for making the arrays and methods for using them, among others. In some embodiments known proteins representing at least 50% of the loci in the human genome are arrayed in known positions on a support. In some embodiments arrays are made of proteins purified from cell lysates by affinity binding to the support. In some embodiments protein arrays are used to decode the binding specificity of antibodies. In some embodiments protein arrays are used to diagnose auto-immune disorders. Many other embodiments and general features are disclosed.

Claims23

Dependent claims

A method for making a protein array, comprising applying lysates L1 through Ln comprising proteins P1 through Pn to positions S1 through Sn on a support,

wherein each lysate Lx is of cells Cx, comprising protein Px expressed therein via exogenous DNA Dx and is applied to position Sx,
wherein
P1 through Pn are all different from one another,
S1 through Sn are all different from one another,
n is an integer greater than 1 and
x is an integer from 1 to n.

3 more elements

Dependencies are inferred from the claim text.

Facts

Application

13/497,895Filed 23 Sep 2010

Priority

25 Sep 2009

Published

13 Sep 2012

Term ends

Not estimatedThe source records don’t support an estimate.

Classification2 codes

G01N 33/6845
General methods of protein analysis not limited to specific proteins or families of proteins: methods of identifying protein-protein interactions…
G01N
Investigating or analysing materials by determining their chemical or physical properties

Protein arrays and uses thereof

Life of this patent

Protein arrays and uses thereof

Life of this patent

Abstract

Claims23