Methods and compositions for diagnosis and prognosis of renal injury and renal failure

A method for evaluating renal status in a subject, comprising:

• obtaining a body fluid sample from a subject selected for evaluation based on a determination that the subject is at risk of a future or current acute renal injury;
• performing one or more analyte binding assays configured to detect one or more biomarkers selected from the group consisting of C Cathepsin B, Renin, soluble Dipeptidyl Peptidase IV, soluble Neprilysin, Beta-2-microglobulin, soluble Carbonic anhydrase IX, and C-X-C motif chemokine 2 by introducing the body fluid sample obtained from the subject into an assay instrument which, for each analyte binding assay performed, (i) contacts all or a portion of the body fluid sample with a specific binding reagent which specifically binds the biomarker being assayed, (ii) and generates an assay result indicative of binding of the biomarker being assayed to the specific binding reagent;
• displaying the assay result(s) generated by the assay instrument in a human-readable form;
• and
• correlating the assay result(s) to the renal status of the subject.