Flow cytometry method through the control of fluorescence intensities

Abstract

Provided is a flow cytometry method including adjusting cell populations targeted by antibodies conjugated with a same-color fluorochrome to show different fluorescence intensities according to types of the antibodies. Unlike a conventional flow cytometry method capable of classifying a positive and negative of one target using one antibody per color, the flow cytometry method adjusts several types of antibodies conjugated with a single-color fluorochrome to respectively show different fluorescence intensities, or adjusts the amounts of antibodies conjugated with a fluorochrome differently according to types of the antibodies, thereby classifying a positive and negative of multiple targets using one color. Accordingly, even when a current flow cytometer capable of classifying a limited number of colors is used, it is possible to classify a variety of cell populations to be clinically examined.

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