Methods and systems associated with detection of fatty acid elongation in a cell
OwnerJanek SZYCHOWSKI, David A. TIRRELL +1inventors
InventorsJanek SZYCHOWSKI, David A. TIRRELL, Sungjin Park
Life of this patent
Estimating the term…
Abstract
Methods and systems to identify compounds capable of altering a fatty acid elongation pathway and for identifying conditions under which fatty acids elongation can occur in a cell are described. The methods and systems comprise labeled fatty acid precursors and cells capable of elongating fatty acids. Methods for providing suitable components of an assay for identifying compounds capable of altering a fatty acid elongation pathway are described.
Claims28
A method to identify a compound capable of altering a fatty acid elongation pathway, the method comprising:
- contacting a candidate compound and a fatty acid precursor comprising a label with a cell comprising the enzymes required in the fatty acid elongation pathway, the contacting performed for a time and under condition to allow elongation of the fatty acid precursor comprising the label through the fatty acid elongation pathway and to allow interference of the candidate compound with the fatty acid elongation pathway;
- and
- detecting the label following the contacting.
A method to determine an effective concentration of one or more compounds capable of altering a fatty acid elongation pathway, the method comprising:
- contacting the one or more compounds at a first concentration and a labeled fatty acid precursor with a cell comprising the enzymes required in the fatty acid elongation pathway, the contacting performed for a time and under condition to allow a first elongation of the labeled fatty acid precursor through the fatty acid elongation pathway and to allow interference of the one or more compound at the first concentration with the fatty acid elongation pathway;
- detecting a first labeling signal associated with the labeled fatty acid precursor following the first elongation, the first labeling signal associated with the first concentration, the detecting performed to determine concentration effective for altering a fatty acid elongation pathway.
A method to identify a cell of capable of elongating an exogenous fatty acid, the method comprising:
- contacting a candidate cell and a fatty acid precursor comprising a label, the contacting performed for a time and under condition to allow elongation of the labeled fatty acid precursor through the fatty acid elongation pathway;
- and
- detecting the label following the contacting.
A method to identify a value or a range of values of a parameter associated with ability of a cell of elongating an exogenous fatty acid, the method comprising:
- contacting the cell and a fatty acid precursor comprising a label, the contacting performed for a time and under condition to allow elongation of the labeled fatty acid precursor through the fatty acid elongation pathway, the conditions comprising a first value of the parameter;
- detecting a first labeling signal from the label, the first labeling signal associated with the first value of the parameter, the detecting performed to determine the value of the parameter associated with ability of the cell of elongating the exogenous fatty acid.
Dependencies are inferred from the claim text.