Protein matrix vaccines of improved immunogenicity
OwnerKevin P. Killen, Thomas J. Griffin, IV +1inventors
InventorsKevin P. Killen, Thomas J. Griffin, IV, Ann Thanawastien
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Abstract
The present invention relates to immunogenic compositions containing an antigen of interest entrapped with a crosslinked carrier protein matrix, methods of making such vaccines, and methods of vaccine administration, wherein the immunogenicity of the protein matrix, and hence its effectiveness as a vaccine, is improved by controlling or selecting the particle size of the protein matrix particles to eliminate low molecular weight particles, e.g., less than 100 nm in diameter.
Claims20
An immunogenic composition comprising (1) an antigen of interest and (2) a carrier protein, wherein said carrier protein is crosslinked to form a protein matrix, said antigen of interest is entrapped by said protein matrix, and said composition is comprised of protein matrix particles having a mean particle size greater than 100 nm diameter.
A method of making an immunogenic composition comprising (i) mixing an antigen of interest with a carrier protein to form a mixture and (ii) crosslinking said carrier protein to form a carrier protein matrix entrapping said antigen of interest, wherein no more than 50% of said antigen of interest is crosslinked to said carrier protein in said composition, and (iii) eliminating from the resulting composition protein matrix particles having a mean particle size diameter of less than 100 nm.
A method of making a protein matrix vaccine composition comprising (i) mixing an antigen of interest with a carrier protein and (ii) initiating a crosslinking reaction with a crosslinking agent that crosslinks functional groups on said carrier protein, and (iii) selecting from said reaction mixture protein matrix particles having a mean particle size diameter of greater than 100 nm.
Dependencies are inferred from the claim text.