Microfluidic Cytochemical Staining System
AssigneeColorado State University Research Foundation
InventorsCharles Henry, Matthew Feirer, Meghan Mensack
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Abstract
The present invention provides devices and associated methods to facilitate multiplexed staining on a slide. The use of multiplexed staining on a single slide allows for more accurate disease diagnosis while also conserving sample. A PDMS based cytology overlay was created that is capable of coupling on-chip morphological, cytochemical, and immunological staining. Morphological and cytochemical staining of blood smears, along with immunostaining of lymph node imprints, was carried out to demonstrate the application of this microfluidic cytology chip (μCC) device.
Claims18
A microfluidic overlay device comprising a substantially planar surface dimensioned to interact with a surface of a microscopic slide, the substantially planar surface having a first end with a plurality of holes and a second end with one or more holes and a plurality of channels, each channel of the plurality of channels connecting one of the plurality of holes at the first end to one of the one or more holes at the second end, wherein the plurality of holes at the first end forms a plurality of inlets and the one or more holes at the second end forms one or more outlets and the plurality of channels forms a sealed conduit between the holes at the first end and the holes at the second end when the microfluidic overlay device is coupled to a microscopic slide.
A microfluidic overlay device comprising a substantially planar sheet having opposing ends with a plurality of holes at a first end, at least one hole at an opposing end and a plurality of channels singularly linking each of the plurality of holes at the first end with the at least one hole at the opposing end, wherein the plurality of holes at the first end form inlets and the at least one hole at an opposing end forms an outlet when overlay device is coupled to a complementary planar surface containing a sample and the plurality of channels provide sealed fluidic communication between the inlets and the at least one outlet when the overlay device is coupled to a complementary planar surface containing a sample.
Dependencies are inferred from the claim text.