Herbicide resistant organisms
AssigneeSapphire Energy, Inc.
InventorsTanya Joy Shin, Yan Poon, Melisa C. Low, Shane Edward Hopkins, Alvin Aram Cho
Life of this patent
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Abstract
Disclosed herein are transformed non-vascular photosynthetic organisms that are herbicide resistant, nucleotides and vectors useful in conducting such transformations, and transformed strains produced by such transformations.
Claims21
1-168. (canceled)
A non-vascular photosynthetic organism comprising at least one mutation in a nucleotide sequence of any one of SEQ ID. NO. 74, SEQ ID. NO. 86, SEQ ID NO. 102, SEQ ID NO. 62, SEQ ID NO. 63, SEQ ID NO. 64, SEQ ID NO. 65, SEQ ID NO. 66, SEQ ID NO. 67, SEQ ID NO. 68, SEQ ID NO. 69, SEQ ID NO. 70, SEQ ID NO. 71, SEQ ID NO. 72, SEQ ID NO. 85, SEQ ID NO. 119, SEQ ID NO. 120, SEQ ID NO. 121, SEQ ID NO. 122, SEQ ID NO. 123, SEQ ID NO. 124, SEQ ID NO. 125, SEQ ID NO. 126 and SEQ ID NO. 127 or a sequence having at least 95% identity thereto;
- wherein the at least one mutation comprises one or more nucleotide additions, deletions or substitutions;
- and wherein the non-vascular photosynthetic organism has an increased growth rate in the presence of a glyphosate herbicide as compared to a non-vascular photosynthetic organism without the at least one mutation.
A genetically modified non-vascular photosynthetic organism comprising at least one RNAi agent, the at least one RNAi agent comprising an antisense nucleotide sequence that is complementary to mRNA transcribed from any one of SEQ ID NO. 74, SEQ ID NO. 86, SEQ ID NO. 102, SEQ ID NO. 62, SEQ ID NO. 63, SEQ ID NO. 64, SEQ ID NO. 65, SEQ ID NO. 66, SEQ ID NO. 67, SEQ ID NO. 68, SEQ ID NO. 69, SEQ ID NO. 70, SEQ ID NO. 71, SEQ ID NO. 72, SEQ ID NO. 85, SEQ ID NO. 119, SEQ ID NO. 120, SEQ ID NO. 121, SEQ ID NO. 122, SEQ ID NO. 123, SEQ. ID NO. 124, SEQ. ID NO. 125, SEQ ID NO. 126, and SEQ. ID NO. 127 or a sequence having at least 95% identity thereto;
- and wherein the non-vascular photosynthetic organism has an increased growth rate in the presence of a glyphosate herbicide as compared to a non-vascular photosynthetic organism not modified with the at least one RNAi agent.
Dependencies are inferred from the claim text.