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US 2012/0084015 A1Published application
Imaging Method and Use Thereof
OwnerChristoph Riethmüllerinventor
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Abstract
The present invention relates to a method based on atomic force microscopy and the use thereof on biological surfaces. A method is provided to detect the Local Deviational Volume (LDV) of defined subcellular structures irrespective of a biochemical characterisation
Claims2
Method for determining and quantifying the topographical elements of biological surfaces using data received by atomic force microscopy, comprising the steps of
- a. Preparing in vitro a single cell, cellular monolayer or tissue section;
- b. Determining the local deviational volume (LDV) of subcelluar structures in a predefined mask in xy-plane;
- c. Normalizing the positive or negative volume of the predefined area;
- d. Quantifying the local deviational volume;
- e. Analysing the data by comparing them with characteristic topographical elements of a calibrated sample;
- f. Evaluating the quantified structural elements to obtain parameter sets.
3 more elements
2-17. (canceled)
Dependencies are inferred from the claim text.