Biomolecular Labelling Using Multifunctional Biotin Analogues
AssigneeThe University of Nottingham
InventorsNeil R. Thomas, Yang Yong-Qing, William C. Drewe
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Abstract
Novel biotin analogues, such as 2-Azidobiotin, comprising the ureido ring of natural biotin with the thiophene ring, optionally modified, and a modified sidechain having a functional end group, preferably selected from the group consisting of a carboxylic acid, amine, alcohol, thiol, aldehyde and a halide, and at least one bio-orthogonally reactive chemical group located elsewhere in the sidechain. The analogues are used for labelling target structures and biomolecules, such as peptides and proteins in vitro or in vivo.
Claims25
A biotin analogue comprising the ureido ring of natural biotin, optionally a modified thiophene ring and a modified sidechain having a functional end group selected from the group consisting of a carboxylic acid, aldehyde, alcohol, amine, thiol and halide, and at least one bio-orthogonally reactive chemical group located elsewhere in the sidechain, wherein the bio-orthogonally reactive chemical group is selected from the group consisting of an azide, an alkyne, an alkene, a heterocyclic group, a diene group and/or one or more heteroatoms selected from S, N, Se, P and
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A biotin analogue as claimed in 3 having the following general formula:
- wherein R has a functional end group selected from the group consisting of a carboxylic acid, aldehyde, alcohol, amine, thiol and halide and includes at least one second functional group selected from the group consisting of an azide, an alkyne, an alkene, a diene, a heterocyclic ring and/or one or more heteroatoms selected from S, N, Se, P and O located elsewhere on the sidechain.
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A biotin analogue having a structure selected from the group consisting of:
16-17. (canceled)
A biotin analogue having the structure:
- wherein X is CH2, O, NH or C═O and R has a functional end group and includes at least one second functional group selected from the group consisting of an azide, an alkyne, an alkene, a diene, a heterocyclic ring and/or one or more heteroatoms selected from S, N, Se, P and O located elsewhere on the sidechain.
A biotin analogue having the structure:
- wherein X is CH2, O, NH or C═O and Y is N, CH or S.
A biotin analogue having the structure:
- wherein X is CH2, O, NH or C═O and Y is N, CH or S.
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Dependencies are inferred from the claim text.