Compositions Containing HC-HA Complex and Methods of Use Thereof
AssigneeTissuetech, Inc.
InventorsScheffer Tseng, Hua He
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Abstract
Disclosed herein, in certain embodiments, is an HC•HA complex comprising hyaluronan and a heavy chain of IαI, wherein the transfer of the heavy chain of IαI is catalyzed by TSG-6. Further disclosed herein, in certain embodiments, is an HC•HA complex comprising hyaluronan and a heavy chain of IαI, wherein the transfer of the heavy chain of IαI is catalyzed by the TSG-6 like protein. Additionally, disclosed herein are methods of manufacturing said complex and methods of use thereof
Claims31
A reaction mixture comprising:
- a. HA;
- b. HC1 and HC2 of IαI, wherein at least one of HC1 and HC2 is optionally recombinant;
- and
- c. TSG-6 or TSG-6 like protein, wherein the TSG-6 or TSG-6 like protein is optionally recombinant.
A purified rcHC•HA complex, wherein the rcHC•HA complex is substantially free of any amniotic material.
A purified rcHC•HA complex, obtained by a process comprising:
- a. providing a reaction mixture comprising:
- i. HA;
- ii. HC1 and HC2 of IαI, wherein at least one of HC1 and HC2 is optionally recombinant;
- and
- iii. TSG-6 or TSG-6 like protein, wherein the TSG-6 or TSG-6 like protein is optionally recombinant;
- wherein at least one of HA, HC1, HC2, TSG-6, TSG-6 like protein is optionally generated by a plurality of cells present in the reaction mixture;
- b. incubating the reaction mixture for a period of time sufficient to produce rcHC•HA complex;
- and
- c. isolating and purifying the rcHC•HA complex.
A purified nHC•HA complex obtained by:
- a. homogenizing chorionic membrane such that it is suitable for extraction of an HC•HA complex;
- and
- b. extracting HC•HA complex by a method selected from:
- chromatography, gel filtration, centrifugation, or differential solubility, ethanol precipitation, or combinations thereof.
A pharmaceutical composition, comprising
- a. rcHC•HA complex;
- and
- b. a pharmaceutically-acceptable excipient.
A method of producing an rcHC•HA complex, comprising
- a. providing a reaction mixture comprising:
- i. HA;
- ii. HC1 and HC2 of IαI, wherein at least one of HC1 and HC2 is optionally recombinant;
- and
- iii. TSG-6 or TSG-6 like protein, wherein the TSG-6 or TSG-6 like protein is optionally recombinant;
- wherein at least one of HA, HC1, HC2, TSG-6, TSG-6 like protein is optionally generated by a plurality of cells in the reaction mixture;
- and
- b. incubating the reaction mixture for a period of time sufficient to produce HC•HA complex.
A method of preventing or reversing scarring in a subject in need thereof, comprising administering to the subject an rcHC•HA complex, an nHC•HA complex isolated from chorionic membrane, or a combination thereof.
Use of an rcHC•HA complex, an nHC•HA complex isolated from chorionic membrane, or a combination thereof to reduce or prevent scarring.
A method of preventing or reducing inflammation in a subject in need thereof, comprising administering to the administering to the subject an rcHC•HA complex, an nHC•HA complex isolated from chorionic membrane, or a combination thereof.
Use of an rcHC•HA complex, an nHC•HA complex isolated from chorionic membrane, or a combination thereof to reduce or prevent inflammation.
A method of preventing or reducing angiogenesis in a subject in need thereof, comprising administering to the subject an rcHC•HA complex, an nHC•HA complex isolated from chorionic membrane, or a combination thereof.
Use of an rcHC•HA complex, an nHC•HA complex isolated from chorionic membrane, or a combination thereof to reduce or prevent angiogenesis.
A method of preventing transplant rejection in a transplant recipient, comprising administering to the transplant recipient an HC•HA complex comprising hyaluronan and a heavy chain of IαI to a subject in need thereof.
Use of an rcHC•HA complex, an nHC•HA complex isolated from chorionic membrane or amniotic membrane, or a combination thereof to prevent transplant rejection.
Dependencies are inferred from the claim text.