Filter Medium For Leukocyte Removal
AssigneeChung Yuan Christian University
InventorsYung Chang, Ruoh-Chyu Ruaan, Ging-Ho Hsiue
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Abstract
The present invention discloses a filter medium for removing leukocytes from a leukocyte-containing sample, comprising: a substrate and a first polymer. The first polymer forms cross-linked networks or polymer brushes on the substrate and comprising charged groups or latent charged groups to control the electrical characteristic of the first polymer. The surface of the substrate formed with the first polymer possesses a specific charge distribution having charged domains and zwitterionic non-charged domains.
Claims35
A filter medium for removing leukocytes from a leukocyte-containing sample, comprising:
- a substrate;
- and
- a first polymer, forming cross-linked networks or polymer brushes on the substrate and comprising charged groups or latent charged groups;
- wherein the surface of the substrate formed with the first polymer possesses a specific charge distribution having charged domains and zwitterionic non-charged domains so that leukocytes are removed from the leukocyte-containing sample and the sample does not form clots when passing through the substrate.
A method for selectively removing leukocytes from a leukocyte-containing sample, comprising:
- providing a filter medium wherein the filter medium comprises a substrate and a first polymer formed on the substrate, the first polymer forms cross-linked networks or polymer brushes on the substrate and comprises charged groups or latent charged groups to control the electrical characteristic of the first polymer such that the surface of the substrate formed with the first polymer possesses a specific charge distribution having charged domains and zwitterionic non-charged domains;
- and
- having the leukocyte-containing sample pass through the filter medium to obtain a filtered solution.
A method for manufacturing a filter medium for removing leukocytes, comprising:
- providing a substrate;
- performing surface treatment to the substrate;
- and
- forming a first polymer on the surface of the substrate by thermal induced radical polymerization or surface initiated atom transfer radical polymerization;
- wherein the first polymer is formed by polymerization of a monomer containing zwitterionic groups and a monomer containing positively charged groups, polymerization of a monomer containing zwitterionic groups and a monomer containing negatively charged groups, or polymerization of a monomer containing positively charged groups and a monomer containing negatively charged groups.
Dependencies are inferred from the claim text.