US 2012/0030842 A1 · METHODS OF PRODUCING GABA

Abstract

The present invention describes an alternative approach to increase GABA production in prokaryotes or eukaryotes, namely by the insertion of the putrescine catabolic pathway in organisms where the pathway does not exist or has not clearly been identified. The invention describes methods for the use of polynucleotides that encode functional putrescine aminotransferase (PAT) and gamma-aminobutyricaldehyde dehydrogenase (GABAlde DeHase) polypeptides in plants to increase GABA production. The preferred embodiment of the invention is in plants but other organisms may be used. Changes in GABA availability will improve growth and increase tolerance to biotic and abiotic stress.

Claims46

Dependent claims

A cell comprising either:

(a) two expression units comprising
(i) a first expression unit comprising a first promoter operably linked to a first polynucleotide which encodes putrescine aminotransferase;
and
ii) a second expression unit comprising a second promoter operably linked to a second polynucleotide which encodes gamma-aminobutyricaldehyde dehydrogenase;
or
(b) a single expression unit which comprising a promoter operably linked to a polynucleotide which encodes either putrescine aminotransferase or both putrescine aminotransferase and gamma-aminobutyricaldehyde dehydrogenase.

3 more elements

A method for producing GABA comprising incorporating into a cell either:

(a) two expression units comprising
(i) a first expression unit comprising a first constitutive promoter operably linked to a first polynucleotide which encodes putrescine aminotransferase;
and
(ii) a second expression unit comprising a second constitutive promoter operably linked to a second polynucleotide which encodes gamma-aminobutyricaldehyde dehydrogenase;
or
(b) a single expression unit which comprises a constitutive promoter operably linked to a polynucleotide which encodes either putrescine aminotransferase or both putrescine aminotransferase and gamma-aminobutyricaldehyde dehydrogenase,
whereby the cell produces GABA.

4 more elements

Dependencies are inferred from the claim text.

Facts

Application

13/274,958Filed 17 Oct 2011

Priority

30 Aug 2007

Published

2 Feb 2012

Term ends

Not estimatedThe source records don’t support an estimate.

Classification9 codes

C12P 13/005
Preparation of nitrogen-containing organic compounds: amino acids other than alpha- or beta amino acids, e.g. gamma amino acids
C12N 9/0004
Enzymes; Proenzymes; Compositions thereof ; Processes for preparing, activating, inhibiting, separating or purifying enzymes: oxidoreductases
C12N 9/1096
Transferases: transferring nitrogenous groups
C12N 15/8243
With non-agronomic quality traits, e.g. for industrial processing; Value added, non-agronomic traits: involving biosynthetic or metabolic…

Family

Application60/968,964Us provisional application · filed 30 Aug 2007

Application60/986,640Us provisional application · filed 9 Nov 2007

Application12/201,648Continuation in part · filed 29 Aug 2008

Application13/095,243Continuation in part · filed 27 Apr 2011

Application13/274,958Continuation in part

US 2009/0077693 A1Published application

US 2011/0203019 A1Published application

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