Methods for differentiating and monitoring parathyroid and bone status related diseases

An assay useful for the determination of whole PTH1-84 while avoiding detection of PTH7-84 utilizing an antibody produced by the following method:

• a) administering a first peptide or protein immunogen to a host animal to induce antibody production against said first peptide or protein immunogen in said host animal, said first peptide or protein immunogen being human, rat, bovine, or porcine PTH1-84;
• b) monitoring antibody titer produced by said administration of said first peptide or protein immunogen to said host animal;
• c) extracting antiserum produced in said host animal by said administration of said first peptide or protein immunogen;
• and
• d) purifying said antiserum and selecting an antibody from said antiserum extracted in step c) by affinity chromatography utilizing a second peptide or protein immunogen, which is human PTH1-8, rat PTH1-8, or a peptide having at least four amino acids in the common sequence of human and rat PTH1-8.