Method of utilizing the pts gene and anti-sense ads to increase patchouli alcohol content in artemisia annua l.
AssigneeFirmenich Aromatics (China) Co., Ltd.
InventorsKe Xuan Tang, Yue Yue Wang, Yue Li Tang, Dong-Fang Chen
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Abstract
The invention relates to a method of utilizing the pts gene and antisense ads to increase patchouli alcohol content in Artemisia annua L. plants. Using transgenic Artemisia annua L. plants, the method of the invention consistently increases the patchouli alcohol content in those plants, thus laying down a solid foundation for large-scale production of patchouli alcohol and other secondary metabolites such as terpenes other than artemisinin.
Claims5
A method of utilizing the pts gene and antisense ads to increase patchouli alcohol content in Artemisia annua L., said method comprising
- a) obtaining the complete open reading frame sequence of artemisinin biosynthesis pathway key enzyme ads gene, the complete open reading frame sequence of the patchouli alcohol biosynthesis pathway key enzyme pts gene, and the tp fragment of the plastid targeted signal peptide of Arabidopsis;
- b) constructing plant expression vectors that contain antisense ads and the plastid targeted pts gene by linking the ads gene obtained in Step a) backwards and the plastid targeted pts gene forwards, respectively, to transcription regulatory sequences;
- c) transforming Agrobacterium tumefaciens simultaneously with expression vectors containing antisense ads and the plastid targeted pts gene to obtain Agrobacterium tumefaciens for transforming Artemisia annua L. plants;
- d) transforming Artemisia annua L. plants with the vector-transformed Agrobacterium tumefaciens obtained in Step c) and analyzing the transformed plants with PCR to obtain transgenic Artemisia annua L. plants;
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- e) determining the patchouli alcohol content in the transgenic Artemisia annua L. plants.
Dependencies are inferred from the claim text.