Detection and typing of bacterial strains
AssigneeDupont Nutrition Biosciences Aps
InventorsW. Michael Russell, Rodolphe Barrangou, PHilippe Horvath
Life of this patent
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Abstract
Methods for the detection and typing of bacterial strains from food products and dietary supplements, environmental samples, in vivo/in vitro samples, and for studying the natural diversity of the species are disclosed. Potential applications also include product development and/or detection and differentiation of new bacterial strains.
Claims11
1-20. (canceled)
An isolated nucleic acid molecule selected from the group consisting of:
- a) a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:1, or a complement thereof;
- b) a nucleic acid molecule comprising a nucleotide sequence having at least 75% sequence identity to the nucleotide sequence of SEQ ID NO:1, or a complement thereof;
- c) a nucleic acid molecule comprising 1-140 repeats of at least one of the nucleotide sequences set forth in SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:40, SEQ ID NO:41, SEQ ID NO:42, SEQ ID NO:43, SEQ ID NO:44, SEQ ID NO:45, SEQ ID NO:46, SEQ ID NO:47, SEQ ID NO:48, or a variant thereof.
A polymerase chain reaction primer, the nucleotide sequence of which comprises SEQ ID NO:49, or a variant thereof.
A polymerase chain reaction primer, the nucleotide sequence of which comprises SEQ ID NO:50, or a variant thereof.
A method for typing a Lactobacillus species having a Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) region, comprising:
- a) obtaining a sample comprising said Lactobacillus;
- b) amplifying a region of DNA comprising said CRISPR region or a fragment thereof in said sample to create amplified DNA.
Dependencies are inferred from the claim text.