Ambient stored blood plasma expanders
AssigneeWake Forest University Health Sciences
InventorMark E. Van Dyke
Life of this patent
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Abstract
A liquid plasma expander or resuscitation fluid composition for use in a subject in need thereof, comprising, consisting of, or consisting essentially of: (a) a keratin derivative (preferably alpha keratose, gamma keratose, or combinations thereof, and with basic alpha keratose preferred over acidic alpha keratose); and (b) an electrolyte solution, with the keratin derivative solubilized in the electrolyte solution to form a homogeneous liquid composition. Blood substitutes formed therefrom and methods of making and using the same are also described.
Claims16
1-16. (canceled)
A method of increasing the volume of available blood substitute for treatment in a subject in need thereof, comprising:
- obtaining a volume of donor blood;
- determining the hematocrit (Hct) of said donor blood;
- isolating red blood cells (RBCs) from said donor blood;
- and
- diluting said isolated RBCs with a plasma expander to a final Hct of not less than 10% but not greater than 70%, wherein said plasma expander consists essentially of:
- (a) from 0.1 to 10 percent by weight of basic alpha keratose;
- said basic alpha keratose having an average molecular weight of from 10 to 100 kilodaltons;
- (b) from 0 to 5 percent by weight of gamma keratose;
- and
- (c) from 90 to 99.9 percent by weight of an electrolyte solution;
- with said basic alpha keratose and said gamma keratose solubilized in said electrolyte solution to form a homogeneous liquid composition having:
- (i) a pH of 7-8;
- (ii) an osmolarity of 200 to 500 milliosmoles/Liter;
- and
- (iii) a viscosity of 2 to 20 centipoise at a temperature of 37 degrees Celsius as determined with a Brookfield viscometer having a cone and plate geometry with a cone angle of 0.02 radians at a constant frequency of 30 rotations per minute.
Dependencies are inferred from the claim text.