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US 2011/0300181 A1Published application
Purification of vaccinia virus- and recombinant vaccinia virus-based vaccines
AssigneeBavarian Nordic A/S
InventorsRene DJURUP, Sara Post Hansen
Life of this patent
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Abstract
The present invention relates to methods for purification of Vaccinia viruses (W) and/or Vaccinia virus (W) particles, which can lead to highly pure and stable virus preparations of predominantly biologically active viruses. The invention encompasses purifying a virus preparation in a sterilized way with high efficiency and desirable yield in terms of purity, biological activity and stability, aspects advantageous for industrial production.
Claims21
1-30. (canceled)
A method for the aseptic purification of biologically active Vaccinia virus comprising:
- i) aseptically loading a solid-phase matrix, to which a ligand is attached, with a Vaccinia virus contained in a liquid-phase culture, wherein the ligand is a glucosamine glycan (GAG) ligand;
- ii) aseptically washing the matrix;
- and
- iii) aseptically eluting the virus.
1 more element
Dependencies are inferred from the claim text.