Methods and compositions for diagnosis and treatment of cancer
AssigneesGanymed Pharmaceuticals AG, Johannes Gutenberg-Universitat Mainz
InventorsUgur Sahin, Ozlem Tureci, Michael Koslowski, Gerd Helftenbein, Korden Walter, Stefan Wöll, Gabriela-Elena Oprea
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Abstract
The present invention relates to the identification of nucleic acid and amino acid sequences that are characteristic of tumor tissues such as ovarian tumor and lung tumor tissues and which represent targets for therapy or diagnosis of tumor diseases in a subject.
Claims30
A pharmaceutical composition comprising an inhibitor of expression and/or activity of a tumor antigen, wherein said tumor antigen comprises an amino acid sequence encoded by a nucleic acid which comprises the nucleic acid sequence according to SEQ ID NO:
- 1 of the sequence listing or a variant of said nucleic acid sequence.
A pharmaceutical composition comprising a ligand of a tumor antigen or a ligand of a nucleic acid encoding a tumor antigen, wherein said tumor antigen comprises an amino acid sequence encoded by a nucleic acid which comprises the nucleic acid sequence according to SEQ ID NO:
- 1 of the sequence listing or a variant of said nucleic acid sequence, and wherein said ligand of a tumor antigen or ligand of a nucleic acid encoding a tumor antigen is attached to one or more therapeutic effector moieties, said therapeutic effector molecule preferably being a radiolabel, cytotoxin or cytotoxic enzyme.
A pharmaceutical composition comprising one or more agents selected from the group consisting of:
- (i) a peptide comprising the amino acid sequence of a tumor antigen or of a tumor antigen peptide derived from said tumor antigen, or a derivative of said peptide,
- (ii) a nucleic acid which codes for a peptide comprising the amino acid sequence of a tumor antigen or of a tumor antigen peptide derived from said tumor antigen, or a derivative of said nucleic acid,
- (iii) a host cell which codes for a peptide comprising the amino acid sequence of a tumor antigen or of a tumor antigen peptide derived from said tumor antigen,
- (iv) a virus which codes for a peptide comprising the amino acid sequence of a tumor antigen or of a tumor antigen peptide derived from said tumor antigen,
- (v) a cell presenting a peptide comprising the amino acid sequence of a tumor antigen peptide derived from a tumor antigen, or a derivative of said peptide,
- (vi) an antibody or T cell receptor which binds to a peptide comprising the amino acid sequence of a tumor antigen or of a tumor antigen peptide derived from said tumor antigen,
- (vii) an immunoreactive cell sensitized in vitro to recognize a peptide comprising the amino acid sequence of a tumor antigen or of a tumor antigen peptide derived from said tumor antigen, and
- (viii) an effector cell or stem cell transduced with a nucleic acid encoding a T cell receptor that recognizes a peptide comprising the amino acid sequence of a tumor antigen or of a tumor antigen peptide derived from said tumor antigen,
- wherein said tumor antigen comprises an amino acid sequence encoded by a nucleic acid which comprises the nucleic acid sequence according to SEQ ID NO:
- 1 of the sequence listing or a variant of said nucleic acid sequence and said tumor antigen peptide comprises an amino acid sequence substantially corresponding to the amino acid sequence of a fragment of said tumor antigen.
A method for diagnosis, detection or monitoring of a tumor disease comprising the detection of and/or determination of the quantity of one or more parameters selected from the group consisting of:
- (i) a nucleic acid which codes for a peptide comprising the amino acid sequence of a tumor antigen,
- (ii) a peptide comprising the amino acid sequence of a tumor antigen or of a tumor antigen peptide derived from said tumor antigen,
- (iii) an antibody which binds to a peptide comprising the amino acid sequence of a tumor antigen or of a tumor antigen peptide derived from said tumor antigen,
- (iv) a T cell that recognizes a peptide comprising the amino acid sequence of a tumor antigen or of a tumor antigen peptide derived from said tumor antigen and/or
- (v) a cell which presents a peptide comprising the amino acid sequence of a tumor antigen peptide derived from a tumor antigen,
- in a biological sample isolated from a patient,
- wherein said tumor antigen comprises an amino acid sequence encoded by a nucleic acid which comprises the nucleic acid sequence according to SEQ ID NO:
- 1 of the sequence listing or a variant of said nucleic acid sequence and said tumor antigen peptide comprises an amino acid sequence substantially corresponding to the amino acid sequence of a fragment of said tumor antigen.
A method for detecting circulating tumor cells in a patient comprising the detection of and/or determination of the quantity of (i) a nucleic acid which codes for a peptide comprising the amino acid sequence of a tumor antigen, and/or (ii) a peptide comprising the amino acid sequence of a tumor antigen or of a tumor antigen peptide derived from said tumor antigen, in a biological sample containing or suspected of containing disseminating or circulating tumor cells or metastatic tumor cells isolated from said patient,
- wherein said tumor antigen comprises an amino acid sequence encoded by a nucleic acid which comprises the nucleic acid sequence according to SEQ ID NO:
- 1 of the sequence listing or a variant of said nucleic acid sequence and said tumor antigen peptide comprises an amino acid sequence substantially corresponding to the amino acid sequence of a fragment of said tumor antigen.
A method of detecting metastatic ovarian cancer cells or metastatic lung cancer cells in a patient comprising the detection of and/or determination of the quantity of (i) a nucleic acid which codes for a peptide comprising the amino acid sequence of a tumor antigen, and/or (ii) a peptide comprising the amino acid sequence of a tumor antigen or of a tumor antigen peptide derived from said tumor antigen, in a biological sample isolated from a tissue or organ of said patient having a tumor wherein the cells when the tissue or organ is free of tumors do not substantially express said nucleic acid or peptide,
- wherein said tumor antigen comprises an amino acid sequence encoded by a nucleic acid which comprises the nucleic acid sequence according to SEQ ID NO:
- 1 of the sequence listing or a variant of said nucleic acid sequence and said tumor antigen peptide comprises an amino acid sequence substantially corresponding to the amino acid sequence of a fragment of said tumor antigen.
A diagnostic test kit which comprises an agent which binds specifically to
- (i) a nucleic acid which codes for a peptide comprising the amino acid sequence of a tumor antigen,
- (ii) a peptide comprising the amino acid sequence of a tumor antigen or of a tumor antigen peptide derived from said tumor antigen,
- (iii) an antibody which binds to a peptide comprising the amino acid sequence of a tumor antigen or of a tumor antigen peptide derived from said tumor antigen,
- (iv) a T cell that recognizes a peptide comprising the amino acid sequence of a tumor antigen or of a tumor antigen peptide derived from said tumor antigen and/or
- (v) a cell which presents a peptide comprising the amino acid sequence of a tumor antigen peptide derived from a tumor antigen,
- wherein said tumor antigen comprises an amino acid sequence encoded by a nucleic acid which comprises the nucleic acid sequence according to SEQ ID NO:
- 1 of the sequence listing or a variant of said nucleic acid sequence and said tumor antigen peptide comprises an amino acid sequence substantially corresponding to the amino acid sequence of a fragment of said tumor antigen, wherein the agent preferably further comprises a detectable label.
An agent which binds to a peptide comprising the amino acid sequence of a tumor antigen or of a tumor antigen peptide derived from said tumor antigen, wherein said tumor antigen comprises an amino acid sequence encoded by a nucleic acid which comprises the nucleic acid sequence according to SEQ ID NO:
- 1 of the sequence listing or a variant of said nucleic acid sequence and said tumor antigen peptide comprises an amino acid sequence substantially corresponding to the amino acid sequence of a fragment of said tumor antigen, wherein the agent preferably is an antibody, more preferably a monoclonal, chimeric, human or humanized antibody, a fragment of an antibody or a synthetic antibody.
Dependencies are inferred from the claim text.