Pathogen Control Genes and Methods of Use in Plants
AssigneeBASF Plant Science GmbH
InventorsPiotr Puzio, Robert Ascenzi, Volker Mittendorf
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Abstract
This invention provides methods for conferring increased pathogen resistance to a plant. Specifically, the invention relates to methods of producing transgenic plants with increased nematode resistance, expression vectors comprising polynucleotides encoding polypeptides with anti-nematode activity, and transgenic plants and seeds generated thereof.
Claims16
An expression vector comprising a polynucleotide selected from the group consisting of:
- a) a polynucleotide having a sequence as defined in SEQ ID NO:
- 1, 3, 5, 7, 9, 135, 137, 139, 141, 143, 145, 147, 149, 151, 153, 155, 157, 159, or 161;
- b) a polynucleotide encoding a polypeptide having a sequence as defined in SEQ ID NO:
- 2, 4, 6, 8, 10, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, or 162;
- c) a polynucleotide having 70% sequence identity to a polynucleotide having a sequence as defined in SEQ ID NO:
- 1, 3, 5, 7, 9, 135, 137, 139, 141, 143, 145, 147, 149, 151, 153, 155, 157, 159, or 161, wherein said polynucleotide confers increased nematode resistance to a plant;
- d) a polynucleotide encoding a polypeptide having 70% sequence identity to a polypeptide having a sequence as defined in SEQ ID NO:
- 2, 4, 6, 8, 10, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, or 162, wherein said polynucleotide confers increased nematode resistance to a plant;
- e) a polynucleotide hybridizing under stringent conditions to a polynucleotide comprising a polynucleotide having a sequence as defined in SEQ ID NO:
- 1, 3, 5, 7, 9, 135, 137, 139, 141, 143, 145, 147, 149, 151, 153, 155, 157, 159, or 161, wherein said polynucleotide confers increased nematode resistance to a plant;
- and
- f) a polynucleotide hybridizing under stringent conditions to a polynucleotide under stringent conditions to a polynucleotide comprising a polynucleotide encoding a polypeptide having a sequence as defined in SEQ ID NO:
- 2, 4, 6, 8, 10, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, or 162, wherein said polynucleotide confers increased nematode resistance to a plant.
The method of claim 20, wherein the plant is selected from the group consisting of pea, pigeonpea, canola, Lotus, sp., Medicago truncatula, alfalfa, soybean, carrot, celery, tomato, potato, cotton, tobacco, pepper, oilseed rape, beet, cabbage, cauliflower, broccoli, lettuce, and Arabidopsis thaliana.
A method of increasing root growth in a crop plant, the method comprising the steps of transforming a crop plant cell with an expression vector comprising a polynucleotide selected from the group consisting of a polynucleotide having a sequence as defined in SEQ ID NO:9, 147, or 149 and a polynucleotide encoding a polypeptide having a sequence as defined in SEQ ID NO:10, 148, and 150;
- and selecting transgenic plants having increased root growth.
Dependencies are inferred from the claim text.