US 2011/0258714 A1 · PHYTASE VARIANTS

Abstract

The present invention relates to a phytase which has at least 74% identity to a phytase derived from Citrobacter braakii and comprises at least one alteration as compared to this phytase. These phytase variants have amended, preferably improved, properties, such as thermostability, temperature profile, pH profile, specific activity, performance in animal feed, reduced protease sensitiliby, and/or an amended glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g. in animal feed and animal feed additives.

Claims29

Dependent claims

A phytase which has at least 74% identity to SEQ ID NO:2 and which comprises at least one alteration as compared to SEQ ID NO:2 in at least one position selected from the following:

1, 2, 3, 4, 5, 31, 41, 46, 52, 53, 55, 57, 59, 74, 76, 82, 84, 91, 99, 100, 104, 105, 107, 109, 111, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 136, 137, 141, 154, 161, 162, 164, 167, 171, 176, 177, 179, 180, 181, 182, 183, 184, 185, 186, 196, 199, 200, 202, 203, 218, 223, 239, 240, 241, 247, 273, 276, 281, 282, 283, 284, 285, 286, 289, 294, 299, 308, 314, 316, 324, 331, 339, 351, 355, 362, 379, 385, 406, 409, 410, and 411;
with the proviso that the phytase is not SEQ ID NO:3, not SEQ ID NO:4, and not SEQ ID NO:6.

Dependencies are inferred from the claim text.

Phytase variants

Life of this patent

Phytase variants

Life of this patent

Abstract

Claims29