Method for the purification of substituted cyclopent-2-en-1-one congeners and substituted 1,3-cyclopentadione congeners from a complex mixture using countercurrent separation

A composition comprising a substantially pure congener or a pharmaceutically acceptable salt thereof, wherein said congener is obtained from a mixture of congeners comprising the steps of:

• a. selecting a congener to be isolated;
• b. dissolving the mixture of congeners in a bi-phasic solvent system specific to the selected congener to be isolated, wherein said bi-phasic solvent system has a partition coefficient from about 0.5 to 5.0;
• c. subjecting the mixture of congeners dissolved in the bi-phasic solvent system to a counter current chromatography;
• and
• d. isolating the selected congener;
• wherein said congener is selected from the group consisting of substituted cyclohexa-2,4-dienones, substituted cyclohexane-1,3,5-triones, substituted cyclopent-2-en-1-ones, and substituted 1,3-cyclopentadiones;
• and
• wherein said composition further comprises a pharmaceutically acceptable excipient.

A method for isolating a congener in substantially pure form from a mixture of congeners comprising the steps of:

• a. preparing a suitable mixture of individual congeners derived from an extract of hops;
• b. dissolving said mixture with a suitable solvent that can be introduced into a counter current separation instrument for the purpose of purification;
• c. collecting a homogenous or partially homogenous solution of individual congeners;
• d. extracting the counter current separation purified congeners into a suitable solvent obtained from step (c);
• or
• e. removing the solvent of the solution obtained in step (c) of a homogenous or partially homogenous solution of an individual congener to render the pure or partially pure congener;
• and
• f. removing the solvent of the solution obtained in step (d) of a homogenous or partially homogenous solution of an individual congener to render the pure or partially pure congener;
• wherein said congener is selected from the group consisting of substituted cyclohexa-2,4-dienones, substituted cyclohexane-1,3,5-triones, substituted cyclopent-2-en-1-ones, and substituted 1,3-cyclopentadiones.

A composition comprising a substantially pure congener or a pharmaceutically acceptable salt thereof, wherein said congener is obtained from a mixture of congeners comprising the steps of:

• a. preparing a suitable mixture of individual congeners derived from an extract of hops;
• b. dissolving said mixture with a suitable solvent that can be introduced into a counter current separation instrument for the purpose of purification;
• c. collecting a homogenous or partially homogenous solution of individual congeners;
• d. extracting the counter current separation purified congeners into a suitable solvent obtained from step (c);
• or
• e. removing the solvent of the solution obtained in step (c) of a homogenous or partially homogenous solution of an individual congener to render the pure or partially pure congener;
• and
• f. removing the solvent of the solution obtained in step (d) of a homogenous or partially homogenous solution of an individual congener to render the pure or partially pure congener;
• wherein said congener is selelected from the group consisting of substituted cyclohexa-2,4-dienones, substituted cyclohexane-1,3,5-triones, substituted cyclopent-2-en-1-ones, and substituted 1,3-cyclopentadiones;
• and
• wherein said composition further comprises a pharmaceutically acceptable excipient.