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US 2011/0257043 A1Published application
Ultra high-throughput opti-nanopore dna readout platform
AssigneePresident and Fellows of Harvard College
InventorsAmit MELLER, Jerome MATHE, John S. EID
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Abstract
Described herein are methods for analyzing polymer molecules. These methods are employed for the high throughput readout of DNA and RNA molecules with single molecule sensitivity. The method of the present invention comprises (1) the electrically controlled unzipping of DNA (or RNA) double strands, and (2) the readout of the molecule's identity (or code) using one or more molecule signal detection.
Claims33
A hybridizable probe polymer-signal molecule composition comprising:
- a hybridizable probe polymer comprising a plurality of hybridizable probe monomers joined together in a sequence corresponding to the nucleotide sequence of a target polynucleotide, wherein one or more hybridizable probe monomers correspond to one or more predetermined nucleotide bases;
- and
- a plurality of signal molecules, wherein each signal molecule is associated with a hybridizable probe monomer, and wherein each signal molecule is bound to a hybridizable probe monomer.
Dependencies are inferred from the claim text.