Bio-field programmable gate array and bio-programmable logic array: reconfigurable chassis construction
AssigneeMassachusetts Institute of Technology
InventorsJonathan William Babb, Ron Weiss, Thomas Knight, Adam Rubin
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Abstract
Aspects of the invention relate to reconfigurable chassis that allow for rapid construction and optimization of biocircuits.
Claims21
A cell comprising a reconfigurable chromosome engineered to express a series of recombination sites, wherein each recombination site has a unique address, and a series of selection markers.
A Bio-Field Programmable Gate Array (BioFPGA) comprising a biological circuit comprising recombinant DNA engineered to express a series of recombination sites, wherein each recombination site has a unique address, and a series of selection markers.
(canceled)
A method comprising:
- providing a cell comprising a reconfigurable chromosome engineered to express a series of recombination sites, wherein each recombination site has a unique address, and a series of selection markers;
- conducting multiplex automated genome engineering (MAGE) on one or more of the recombination sites in the reconfigurable chromosome, thereby generating a reconfigured chromosome;
- providing a plasmid that comprises one or more recombination sites matching the mutated recombination sites on the reconfigured chromosome;
- and
- conducting recombination between the plasmid and the reconfigured chromosome.
A cell comprising a reconfigurable chromosome engineered to express a biological circuit comprising a set of programmable AND gates linked to a set of programmable OR gates, wherein the biological circuit comprises one or more configuration bits that, when mutated, change the functionality of the biological circuit.
A Bio-Programmable Logic Array (BioPLA) comprising a biological circuit comprising recombinant DNA engineered to express a set of programmable AND gates linked to a set of programmable OR gates, wherein the biological circuit comprises one or more configuration bits that, when mutated, change the functionality of the biological circuit.
A method comprising:
- providing a cell comprising a reconfigurable chromosome engineered to express a biological circuit comprising a set of programmable AND gates linked to a set of programmable OR gates, wherein the biological circuit comprises one or more configuration bits that, when mutated, change the functionality of the biological circuit;
- and
- conducting multiplex automated genome engineering (MAGE) on one or more of the configuration bits, thereby changing the functionality of the biological circuit.
Dependencies are inferred from the claim text.