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OwnerJohanna Kate Daniels, Daniel David Palmer +1inventors
InventorsJohanna Kate Daniels, Daniel David Palmer, Richard James Calder
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A method is provided of making meltable beads for use in reactions for the amplification and/or synthesis of a polynucleotide, the method comprising (i) Providing one or more reagents for use in a reaction for the amplification and/or synthesis of a polynucleotide and a substance for promoting the formation of a solid bead; (ii) Providing a reactor device comprising a sample conduit and a first carrier fluid conduit for the flow of immiscible liquids, the sample conduit and first carrier fluid conduit meeting at a junction; (iii) Heating the one or more reagents for use in a reaction for the amplification and/or synthesis of a polynucleotide and the substance for promoting the formation of a solid bead so as to form a liquid comprising the one or more reagents for use in a reaction for the amplification and/or synthesis of a polynucleotide and the substance for promoting the formation of a solid bead in intimate admixture; (iv) Passing the liquid down the sample conduit and a carrier fluid down the first carrier fluid conduit, thus causing the formation of droplets at or downstream of the junction and (v) Causing the formation of solid beads by cooling the droplets. A device for use in such methods
A method of making meltable beads for use in reactions for the amplification and/or synthesis of a polynucleotide, the method comprising
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A microfluidic reactor device for the manufacture of meltable beads, the reactor device comprising:
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A plurality of meltable spherical beads for use in a reaction for the amplification and/or synthesis of a polynucleotide, the beads having a mean diameter of from 50 to 2500 microns, the standard deviation of the diameter of the plurality of beads being no greater than 5% of the mean diameter of the plurality of beads, each of the beads comprising one or more reagents for use in reaction for the amplification and/or synthesis of a polynucleotide
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A plurality of reaction vessels, each reaction vessel containing one or more meltable spherical beads for use in a reaction for the amplification and/or synthesis of a polynucleotide, the beads in the plurality of reaction vessels having a mean diameter of from 50 to 2500 microns, the standard deviation of the diameter of the beads in the plurality of reaction vessels being no greater than 5% of the mean diameter, each of the beads comprising one or more reagents for use in reaction for the amplification and/or synthesis of a polynucleotide.
An aqueous composition for the formation of meltable beads for use in a reaction for the amplification and/or synthesis of a polynucleotide, the composition comprising a substance for promoting the formation of a solid bead and one or more reagents for use in a reaction for the amplification and/or synthesis of a polynucleotide, the composition comprising 0.1 to 10 wt % of the substance for promoting the formation of a solid bead.
A plurality of meltable spherical beads for use in a reaction for the amplification and/or synthesis of a polynucleotide, the plurality of beads being produced using a method comprising:
Dependencies are inferred from the claim text.