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AssigneeUniversity of Virginia Patent Foundation
InventorsRoy Clinton Ogle, Edward A. Botchwey, III, Rebekah A. Neal
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The present invention provides methodologies and parameters for fabrication of the hybrid biomaterial by blending pure laminin or complex extracts of tissues containing laminin with biopolymers such as polycaprolactone (PCL), polylactic/polyglycolic acid copolymer (PLGA) or Polydioxanone (PDO) in fluoroalcohols (HFP, TFA), fabrication of substrates and scaffolds and devices from the hybrid biomaterial in forms such as films, nanofibers by electrospinning or microspheres, and the biological or biomedical use of the material or devices derived from it.
A method for treating an injured nerve, said method comprising contacting said injured nerve with nanofibers electrospun from a mixture of a protein and a polymer, thereby treating an injured nerve.
A method for stimulating cell attachment, proliferation, or migration, said method comprising providing said cell with nanofibers electrospun from a mixture of a protein and a polymer, further wherein at least one growth factor is optionally provided, thereby stimulating cell attachment, proliferation, or migration.
A method for preparing an electrospun nanofiber comprising a mixture of a protein and a polymer, said method comprising obtaining a purified protein, dissolving said purified protein in HFP, obtaining a purified polymer, dissolving said polymer in the HFP comprising protein, loading said solution comprising protein and polymer into a dispensing container comprising a positive lead, subjecting said lead to driving voltage from a power supply, pumping said protein and polymer dissolved in HFP through an opening in said dispensing container, and collecting said protein and polymer mixture dissolved in HFP on a substrate placed on a grounded collector.
An electrospun nanofiber prepared from mixture of a protein and a polymer, wherein the protein and polymer mixture is prepared at a weight to volume total percentage of about 1% to about 70% in HFP and the amount of protein to polymer in the mixture prepared for electrospinning is from about 0.1% to about 70%, weight to weight.
Dependencies are inferred from the claim text.