Methods for Processing Biological Tissues
AssigneeColorado State University Research Foundation
InventorsE. Christopher Orton, Shiori Arai, Carla Maria Ribeiro Lacerda, Leigh Gareth Griffiths
Life of this patent
Estimating the term…
Abstract
Methods are provided for removing or separating the cellular and/or soluble macromolecular component of a biological tissue from the extracellular matrix component of the biological tissue, comprising embedding the biological tissue in an electrically conductive semi-solid or solid supporting medium and applying an electric field to the tissue-medium complex. Additionally, methods are provided for decellularizing a skin fragment.
Claims62
A method for removing or separating the cellular and/or soluble macromolecular component of a biological tissue from the extracellular matrix component of the biological tissue, comprising embedding the biological tissue in an electrically conductive semi-solid or solid supporting medium and applying an electric field to the resulting tissue-medium complex.
A method for processing a biological tissue prior to implantation or transplantation, comprising embedding the biological tissue in an electrically conductive semi-solid or solid supporting medium and applying an electric field to the resulting tissue-medium complex.
A method for preparing a biological tissue scaffold, comprising embedding a biological tissue in an electrically conductive semi-solid or solid supporting medium and applying an electric field to the resulting tissue-medium complex.
A method for decellularizing a skin fragment, comprising the steps of:
- i) treating the skin fragment in a hypertonic solution;
- ii) embedding the skin fragment in an electrically conductive semi-solid or solid supporting medium;
- iii) subjecting the resulting skin fragment-medium complex to electrophoresis;
- and
- iv) washing the skin fragment in a hypotonic or isotonic solution.
Dependencies are inferred from the claim text.