Method to determine state of a cell exchanging metabolites with a fluid medium by analyzing the metabolites in the fluid medium
AssigneeMcGill University
InventorDavid Hugh Burns
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Abstract
The present invention relates to a method for determining the ideal time for and outcome of reproductive health procedures including in vitro fertilization by establishing a correlation between the successful outcome of said procedure and the spectra of a body fluid obtained using a chosen analytical modality for a population of patients, acquiring for a patient a spectrum of the body fluid of the patient using said chosen modality.
Claims25
A method to determine viability of a cell exchanging metabolites with a fluid medium, comprising
- acquiring for at least one cell a spectrum of a fluid medium using a spectrometer with a chosen analytical modality, wherein the at least one cell is exchanging metabolites with said fluid medium;
- and
- generating probability data for viability of the at least one cell using the acquired spectrum and a correlation between viability of cells and spectra of fluid media obtained using the chosen analytical modality for a population of cells exchanging metabolites with said fluid media, wherein the at least one cell is an oocyte, spermatozoid, embryo, cell from a uterine wall or stem cell.
A method for sustaining or growing one or more cells in a culture medium, the method comprising:
- adjusting the culture medium using a state of the cells determined from a spectrum of the culture medium obtained using a chosen analytical modality.
An apparatus for controlling culture of one or more cells growing in vitro in a culture medium, the apparatus comprising:
- a spectral acquisition device for acquiring a spectrum of said culture medium;
- a database of correlation data relating spectral data to a state of said cells;
- a state determination processor generating data representing said state using said correlation data and said spectrum;
- a culture medium controller generating control signals for effecting an adjustment in said culture medium in response to said data representing said state.
Dependencies are inferred from the claim text.