Bluetongue virus recombinant vaccines and uses thereof
AssigneeMerial Limited
InventorsJean-Christophe Audonnet, Xuan Guo, Kevin Cox
Life of this patent
Estimating the term…
Abstract
The present invention encompasses BTV vaccines or compositions. The vaccine or composition may be a vaccine or composition containing BTV antigens. The invention also encompasses recombinant vectors encoding and expressing BTV antigens, epitopes or immunogens which can be used to protect animals, such as ovines, bovines, or caprines, against BTV.
Claims25
A composition comprising a BTV (Bluetongue Virus) antigen and a pharmaceutical or veterinarily acceptable carrier, excipient, adjuvant, or vehicle.
A substantially purified BTV polypeptide expressed in a plant or microalga, wherein the polypeptide comprises:
- a) an amino acid sequence having at least 80% sequence identity to a polypeptide having the sequence as set forth in SEQ ID NO:
- 4, 6, or 10;
- b) a conservative variant of the amino acid sequence as set forth in SEQ ID NO:
- 4, 6, or 10;
- c) an immunogenic fragment comprising at least eight consecutive amino acids of the amino acid sequence as set forth in SEQ ID NO:
- 4, 6, or 10, that specifically binds to an antibody that specifically binds to the amino acid sequence as set forth in SEQ ID NO:
- 4, 6, or 10.
A plasmid comprising a DNA fragment having at least 70% sequence identity to the sequence as set forth in SEQ ID NO:
- 1, 2, 3, 5, 7, 8, or 9.
A stably transformed duckweed plant or culture transformed with a gene expressing a BTV antigen or fragment or variant thereof.
A method of producing a BTV antigen comprising:
- (a) culturing within a duckweed culture medium a duckweed plant or duckweed nodule, wherein the duckweed plant or the duckweed nodule is stably transformed to express the antigen, and wherein the antigen is expressed from a nucleotide sequence comprising a coding sequence for the antigen;
- and (b) collecting the antigen from the duckweed plant or duckweed nodule.
Dependencies are inferred from the claim text.