Method and device for flow cytometry without sheath fluid

Flow cytometry device without sheath fluids for particle characterization comprising:

• an aperture flow-cell (2) at least in part substantially transparent to at least one wavelength of interest, and at least in part substantially insulating to electricity,
• means for transfer of a fluid (3) containing particles (4) through said aperture flow-cell,
• means for measurement of the electrical impedance variation generated by the passage of said particles (4) contained in the fluid (3) through the aperture flow-cell (2),
• means for measurement of at least one optical property of said particles (4), said particles interacting with at least one light beam (7) passing through the aperture flow-cell,
• means for analysis of said measurements of variation in electrical impedance and optical properties,
• characterized in that it comprises:
• means for deducing information on the trajectory of the particles in the aperture flow-cell (2), at least from the analysis of said measurement of the electrical impedance variation, and
• means for using said information on the trajectory at least for the exploitation of said measurements of optical properties during characterization of the particles.