Engineered light-emitting reporter genes and methods of use
AssigneeCobalt Technologies, Inc.
InventorsStacy M. Burns-Guydish, Pamela Reilly Contag
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Abstract
Compositions and methods are provided for enhanced expression of light emitting reporters. Such reporters are used in methods for monitoring cultures for production of target compounds. Additionally, methods are provided for the use of light emitting reporter systems and other reporter systems for selecting desired traits in an organism and for identifying or optimizing culture conditions.
Claims29
1-33. (canceled)
A method for selecting mutants with a desired characteristic that produce an intermediate or end product of a fermentative, synthetic, or metabolic pathway at a higher titer, higher yield, or at a higher ratio over a less desired product, comprising:
- a) mutagenizing a plurality of recombinant cells that comprise a recombinant nucleic acid molecule comprising an expression control sequence operatively linked with a coding nucleotide sequence encoding a reporter;
- b) isolating pure cultures derived from individual mutants;
- c) measuring the expression of the reporter in the cultures;
- and
- d) selecting mutants that have a different expression level of the reporter compared to unmutagenized recombinant cells, wherein the different expression level indicates that the intermediate or end product is produced at the higher titer, yield, or ratio.
(canceled)
Dependencies are inferred from the claim text.