Method for patterning magnetic materials in live cell, method for imaging pattern of magnetic materials, and apparatus used for same

A method for imaging a pattern of magnetic materials in a live cell comprising:

• (a) preparing a plurality of magnetic materials magnetized in a direction of a line of magnetic force by applying a magnetic field, at least one of the magnetic materials being configured into a nanoparticle and the surface of at least one of the magnetic materials being modified;
• (b) preparing a mediator comprising a first linker and a second linker which are subjected to identify whether the linkers bind to each other in a live cell;
• (c) introducing a plurality of the magnetic materials into each live cell, the first linker being associated with the magnetic material;
• (d) providing the live cell with a label capable of imaging a pattern of the magnetic materials in a direction of a line of magnetic force, the second linker being associated with the label;
• (e) allowing a bundle of the lines of magnetic force to pass through the live cell in a direction by applying a focused magnetic field to the live cell;
• (f) aligning a plurality of the magnetic materials in the live cell with the direction of the line of magnetic force by the applied magnetic field;
• (g) identifying a pattern imaged by the label capable of imaging the aligned pattern of the magnetic materials;
• and
• (h) determining that the label has been labeled to the magnetic material in the live cell by binding the second linker to the first linker from the identification of the step (g).

An apparatus used for imaging a pattern of magnetic materials in a live cell comprising:

• a live cell provided with a plurality of magnetic materials magnetized in a direction of a line of magnetic force by applying a magnetic field;
• at least one of the magnetic materials being configured into a nanoparticle;
• the surface of at least one of the magnetic materials being modified;
• and the magnetic material being associated with a first linker of two linkers which are subjected to identify whether the linkers bind to each other in the live cell, and provided with a label capable of imaging a pattern of the magnetic materials, the label being associated with a second linker of two linkers;
• a container for receiving and culturing the live cell;
• an apparatus for allowing a bundle of the lines of magnetic force to pass through the live cell in a direction by applying a focused magnetic field to the live cell;
• and
• a device for monitoring that the label has been labeled to the magnetic material in the live cell by binding the second linker to the first linker after imaging the pattern of the magnetic materials aligned with the direction of the line of magnetic force and the pattern imaged by the label.