Novel method
OwnerBruno Rene Andre, Benoit Paul Suzanne Champluvier +1inventors
InventorsBruno Rene Andre, Benoit Paul Suzanne Champluvier, Bénédicte Van Der Heyden
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Abstract
The present invention relates to a method for degrading host cell nucleic acids associated with a virus or a viral antigen thereof produced by cell culture, the method comprising at least two steps of nucleic acids degradation with a compound selected from i) an endonuclease and ii) a DNA alkylating agent.
Claims28
-44. (canceled)
A method for degrading host cell nucleic acids associated with a virus or a viral antigen thereof produced by cell culture, the method comprising at least two host cell nucleic acids degradation steps with a compound selected from i) an endonuclease and ii) a DNA alkylating agent.
A method for producing a virus or a viral antigen thereof in cell culture comprising the steps of:
- a. providing a population of host cells cultured in a cell culture medium,
- b. inoculating the population of host cells with a virus,
- c. culturing the population of host cells so as to allow the virus to replicate,
- d. collecting the produced virus thereby providing a viral harvest, and
- e. isolating the virus,
- the method further comprising at least two host cell nucleic acids degradation steps with a compound selected from i) an endonuclease and ii) a DNA alkylating agent.
An immunogenic composition comprising a cell culture-produced virus, or a viral antigen thereof, admixed with a suitable pharmaceutical carrier, wherein the residual host cell content of DNA fragments whose length is 300 base pairs is less than 1 ng as measured by quantitative PCR.
An immunogenic composition comprising a cell culture-produced virus, or a viral antigen thereof, admixed with a suitable pharmaceutical carrier, wherein the residual host cell content of DNA fragments whose length is 60 base pairs is less than 1 ng as measured by quantitative PCR.
Dependencies are inferred from the claim text.