Perfusion bioreactors, cell culture systems, and methods for production of cells and cell-derived products
AssigneeBiovest International, Inc.
InventorsMark HIRSCHEL, Darrell P. PAGE, Robert J. WOJCIECHOWSKI
Life of this patent
Estimating the term…
Abstract
The present invention includes perfusion bioreactors, automated cell culture systems, and methods for production of cells and cell-derived products.
Claims26
A perfusion bioreactor, comprising a housing with an inlet port and outlet port, and a cell growth matrix, wherein said cell growth matrix is planar, pleated, or spirally wound around a central core.
A cell culture system for the production of cells and/or cell-derived products, comprising:
- a reusable instrumentation base device incorporating hardware to support a cell culture growth;
- and
- at least one disposable cell cultureware module removably attachable to said instrumentation base device, said module including at least one perfusion bioreactor comprising a housing with an inlet port and outlet port, and a cell growth matrix, wherein said cell growth matrix is planar, pleated, or spirally wound around a central core.
A method for the production of cells and/or cell-derived products, comprising the steps of:
- providing at least one disposable cultureware module, said module including at least one perfusion bioreactor comprising a housing with an inlet port and outlet port, and a cell growth matrix, wherein said cell growth matrix is planar, pleated, or spirally wound around a central core;
- providing a reusable instrumentation base device incorporating hardware to support cell culture growth, said base device including a microprocessor control and a pump for circulating cell culture medium through the bioreactor;
- removably attaching said at least one cultureware module to said instrumentation base device;
- introducing cells into the bioreactor;
- fluidly attaching a source of cell culture medium to said at, least one cultureware module;
- programming operating parameters into the microprocessor control;
- operating the pump to circulate the cell culture medium through the bioreactor to grow cells or cell-derived products therein;
- and optionally, carrying out one or both of the following:
- harvesting the grown cells or cell-derived products from the bioreactor;
- and
- disposing of said at least one cultureware module.
A method for the production of cells and/or cell-derived products, comprising:
- providing at least one least one perfusion bioreactor;
- introducing cells into said bioreactor, and culturing the cells within said bioreactor, wherein said bioreactor comprises a housing with an inlet port and outlet port, and a cell growth matrix, wherein said cell growth matrix is planar, pleated, or spirally wound around a central core.
Dependencies are inferred from the claim text.