Multi-Media Affinity Column to Prevent Leaching of Ligands
AssigneeBio-Rad Laboratories, Inc.
InventorMark A. Snyder
Life of this patent
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Abstract
In an affinity-type purification, ligands dissociated from a packed bed that would otherwise leach into the solution containing the species being purified are captured by a second ligand that resides in a porous barrier downstream from the packed bed, the second ligand exhibiting an affinity-type interaction with the dissociated first ligand with sufficient specificity to avoid the undesired retention by the second ligand of species from the liquid sample or source liquid other than the species sought to be purified in the affinity column.
Claims16
An affinity column for extracting a solute from a liquid sample, said column comprising:
- a flow-through tubular housing defining a flow direction therethrough;
- a packed bed of solid particulate support material with a first ligand immobilized thereon that binds selectively to said solute by affinity-type binding;
- and
- a porous barrier spanning said housing at a site downstream of said packed bed in said flow direction, said porous barrier having a second ligand immobilized thereon that binds selectively by affinity-type binding to molecules of said first ligand or to segments of said molecules upon release of said molecules or segments from said solid particulate support material.
Dependencies are inferred from the claim text.