Method for methylmalonic acid detemination based on alkylative extraction associated to liquid chromatography coupled to mass spectrometry

A method for determination of methylmalonic acid present in biologic samples characterized by the extraction and derivatization of methylmalonic acid followed by the use of mass spectrometry and atmospheric pressure ionization.

A method for determination of presence of methylmalonic acid in biologic samples, according to any previous claim, characterized by preferably using the following instrumentations:

• LC-MS/MS system constituted by a tandem mass spectrometer equipped with ionization probe preferably at atmospheric pressure, preferably on negative mode;
• a liquid chromatography system comprised by pump and sampler, a system control software with mass spectrometer and chromatographic system and data processing.