Regeneration and mass propagation of jatropha curcas through somatic embryogenesis
AssigneeTemasek Life Sciences Laboratory Limited
InventorsLin Cai, Lianghui Ji
Life of this patent
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Abstract
The present invention relates to the field of somatic embryo production, particularly to methods for the regeneration of Jatropha through somatic embryogenesis. More specifically, the present invention relates to a method and media compositions for regeneration of plants of Jatropha curcas. The method is well suited for Jatropha curcas transformation and for producing clonal planting stock useful for large scale Jatropha curcas plantation.
Claims52
A method for regenerating fertile plants of the genera Jatropha through somatic embryogenesis comprising:
- (a) isolating endosperm tissue from Jatropha immature zygotic embryos;
- (b) culturing the endosperm tissue on a solid auxin-containing medium for a period sufficient to induce production of somatic embryos;
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- (c) culturing the somatic embryos on a solid germination medium for a period of time sufficient to geminate the somatic embryos to produce plantlets.
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A method for regenerating fertile plants of the genera Jatropha through somatic embryogenesis comprising:
- (a) culturing an explant of the genera Jatropha on a solid hormone-containing medium for a period sufficient to initiate calli growth;
- (b) culturing the explant or calli on a hormone-free medium for a period sufficient to develop somatic embryos or embryogenic calli;
- (c) culturing the somatic embryos on a solid maturation medium for a period of time sufficient to mature the somatic embryos;
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- (d) culturing the matured somatic embryos on a germination medium for a period of time sufficient to germinate the somatic embryos to produce plantlets.
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A method for establishment of plant somatic embryogenic liquid suspension cultures of the genera Jatropha comprising:
- (a) culturing an explant of the genera Jatropha on a solid hormone-containing medium for a period sufficient to initiate calli growth;
- (b) culturing the explant or calli on a hormone-free medium for a period sufficient to develop somatic embryos or embryogenic calli;
- (c) culturing the embryogenic calli or somatic embryos in a liquid medium to produce somatic embryogenic calli or embryoids;
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- (d) regularly subculturing and size-selecting the somatic embryogenic calli or embryoids.
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Dependencies are inferred from the claim text.