Hematopoietic Fingerprints: Methods of Use
OwnerMargaret A. Goodell, Stuart M. Chambers +3inventors
InventorsMargaret A. Goodell, Stuart M. Chambers, Nathan C. Boles, Kuan-yin K. Lin, Megan P. Tierney
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Abstract
The present invention is related to the discovery that the detection of one or more biomarkers in a body sample can identify hematapoietic progenitors that are precursors to a specific blood cell lineage. The methods of the present invention are also directed to the detection of the dysregulation of these biomarkers as a diagnostic assay for the certain disease states, such as cancer.
Claims32
A method of directing the differentiation of a hematopoietic stem cell (HSC), a hematopoietic progenitor cell (HPC), or a combination thereof, said method comprising introducing into said cell a zinc finger protein (Zfp105), wherein said Zfp105 upregulates expression of a natural killer (NK) cell-specific polynucleotide in said cell, thereby inducing differentiation of said cell into an NK cell.
A method of directing the differentiation of a HSC, a HPC, or a combination thereof, said method comprising introducing into said cell an Ets2, wherein said Ets2 upregulates expression of a monocyte-specific polynucleotide in said cell thereby inducing differentiation of said cell into a monocyte.
A method of directing the differentiation of HSC, a HPC, or a combination thereof, to a B-cell, said method comprising introducing to said cell at least one biomarker selected from the list consisting of Chd7 (chromodomain helicase DNA binding protein 7), Edaradd (EDAR (ectodysplasin-A receptor)-associated death domain, 2210016F16Rik, Dzip1 (DAZ interacting protein 1), and Tbl1x (transducin (beta)-like 1 X-linked).
A method of directing the differentiation of a HSC, a HPC, or a combination thereof, to a cell of the myeloid lineage, said method comprising introducing to said cell at least one biomarker selected from the list consisting of Med8 (mediator of RNA polymerase II transcription, subunit 8 homolog), Med14 (mediator complex subunit 14), GLIS2 (GLIS family zinc finger 2), Tnfaip8l1 (tumor necrosis factor, alpha-induced protein 8-like 1), and Mina (myc induced nuclear antigen).
Dependencies are inferred from the claim text.