Method for isolating renal stem/progenitor cells, renal stem/progenitor cells and therapeutic agent for renal disease
AssigneeBio Link, Incorporated
InventorsTakeshi Sugaya, Naoshi Shinozaki
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AssigneeBio Link, Incorporated
InventorsTakeshi Sugaya, Naoshi Shinozaki
Estimating the term…
It is intended to provide a method for noninvasively isolating human renal stem/progenitor cells, an isolated renal stem/progenitor cells, a therapeutic agent for renal disease, mouse mesenchymal cells which can be used for isolating human renal stem/progenitor cells and a culture supernatant of the same. Renal stem/progenitor cells are isolated by primarily culturing cells contained in the urine of a patient having renal disease in a medium containing mouse mesenchymal cells identified by the deposition number of FERM ABP-10865 or a culture supernatant of the same, staining the obtained primarily cultured cells with Hoechst 33342 and separating a weak-positive or negative fraction.
A method for isolating renal stem/progenitor cells comprising:
Mouse mesenchymal cells identified by the deposition number of FERM ABP-10865 used for isolation of renal stem/progenitor cells from urine of a patient having renal disease.
A culture supernatant obtained by culturing mouse mesenchymal cells identified by the deposition number of FERM ABP-10865 in a serum-containing medium.
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