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OwnerJohn W. Brandis, Kenneth A. Johnsoninventors
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The present disclosure relates to nucleic acids comprising a sequence of SEQ ID NO: 1. The nucleic acid may be an isolated DNA and/or may be in the form of a plasmid or an expression vector. It may also be comprised in a microorganism. The nucleic acid may further comprise sequences that encode a protein. The self-replicating expression plasmid comprising a DNA sequence of the disclosure may be used to produce one or more protein. The production of one or more protein by a plasmid of the disclosure may be controlled by temperature and/or chemical induction. The disclosure also provides methods of obtaining high yields of proteins and methods for purifying such proteins, such as the LdK39 protein or a fragment thereof.
An isolated DNA comprising a sequence of SEQ ID NO:
A recombinant plasmid comprising an isolated DNA comprising a sequence of SEQ ID NO:
A microorganism comprising DNA comprising a sequence of SEQ ID NO:
A self-replicating nucleic acid molecule comprising:
A method of producing at least one protein, comprising inducing expression of the at least one protein using a recombinant plasmid comprising an isolated DNA having a sequence of SEQ ID NO:
A method of producing at least one protein, comprising inducing expression of the at least one protein using a recombinant plasmid comprising an isolated DNA having a sequence of SEQ ID NO:
A protein production system comprising:
A protein production system comprising a self-replicating nucleic acid molecule comprising:
A method for protein purification comprising:
An E. coli-based protein production system comprising:
Dependencies are inferred from the claim text.