Method for continuously detecting glucose concentration in sample, kit thereof and method for using biosensor

A kit for continuously detecting glucose concentration in a sample, comprising:

• a biosensor comprising a transducer and a polysaccharide covered on the surface of the transducer;
• a reactive solution for reacting with the sample, wherein the reactive solution comprises a carbohydrate binding protein solution with a particular concentration;
• and
• a washing solution for refreshing the surface of the biosensor, wherein the washing solution comprises a high concentration glucose solution.

A method for using a biosensor, comprising:

• (a) providing a biosensor comprising a transducer and a polysaccharide covered on the surface of the transducer;
• (b) contacting a mixture of a reactive solution comprising a carbohydrate binding protein solution with a particular concentration and a sample with the biosensor, wherein the carbohydrate is capable of binding to the polysaccharide and glucose;
• (c) detecting the amount of carbohydrate binding proteins bound to the polysaccharide by the biosensor to obtain glucose concentration of the sample;
• and
• (d) refreshing the surface of the biosensor with a washing solution, wherein the washing solution comprises a high concentration glucose solution.