Image reconstruction for unordered microwell plates

An image reconstruction method for unordered microwell plates from disordered or randomized dispenses across multiple microwell plates, comprising:

• creating multiple corresponding dispensing logs identified by a microwell plate identifier;
• capturing digital images of the multiple unordered microwell plates;
• using multiple digital images of unordered microwell plates along with the corresponding dispensing logs information for the image reconstruction;
• selecting a grouping of compound effects for a range of concentration levels that will fit in a single reconstructed digital image;
• establishing one or more rules to govern the reorganization of the digital images;
• reordering the combined dispensing log information identified by a microwell plate identifier;
• creating a reordered log with well input locations reordered by test compound identity and quantity levels dispensed order;
• identifying digital image pixels for each unordered dispensed test well input location;
• creating an ordered reconstructed output digital image for a grouping of compound effects for a range of concentration levels;
• and
• placing copies of the corresponding ordered test well pixels for a selected grouping of compound effects in output locations in a single reconstructed digital image for visualization of effects in the microwell plates in ordered compound concentrations;

An image reconstruction apparatus for unordered microwell plates, comprising:

• one or more digital images configured to show reconstructions of compound effects of unordered microwell plates compound effects;
• a dispensing log configured to list dispensing log information such as the plate identifier, the identity of the compounds being dispensed and the quantity of compound dispensed into each individual test well by location;
• a dispensing log configured to list information used to create a an unordered microwell plate;
• digital image pixels identified by the locations within the unordered dispensed test well images;
• one or more rules to govern the reorganization of the unordered digital image;
• a reordered log with well input locations reordered by test compound identity and quantity levels dispensed order corresponding reordered log well output locations;
• and
• a digital manipulator configured to copy test well digital image pixels from well input locations to reordered output locations to define a reconstructed digital image for visualization of visible compound effects in the microwell plates in ordered compound concentrations.