Bioluminescent protease assay
AssigneePromega Corporation
InventorsMartha O'Brien, Keith V. Wood, Dieter Klaubert, William Daily
Life of this patent
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Abstract
A sensitive bioluminescent assay to detect proteases including caspases, trypsin and tryptase is provided.
Claims13
A method to prepare a carboxy-terminal protected derivative of aminoluciferin, comprising protecting the corresponding acid with a suitable carboxy-protecting group.
The method of claim 3 wherein the acid is protected by a tert-butyl ester.
A method for preparing a compound comprising forming an amide bond between a first amino acid or peptide and the amino group of a solid support bound aminoluciferin;
- and optionally attaching one or more additional amino acids or peptides through peptide bonds to provide the compound.
A method to prepare a compound of formula (I):
- wherein R is a peptide that is a substrate for caspase, which is linked to the remainder of the compound of formula (I) through its C-terminus forming a peptide (amide) bond;
- and R′ is a suitable carboxy-protecting group;
- comprising protecting the corresponding acid, wherein R′ is hydrogen, with a suitable carboxy-protecting group.
A method to prepare a compound of formula (I):
- wherein R is a peptide that is linked to the remainder of the compound of formula (I) through an aspartate group at the C-terminus of the peptide forming a peptide bond;
- and R′ is a suitable carboxy-protecting group;
- comprising protecting the corresponding acid, wherein R′ is hydrogen, with a suitable carboxy-protecting group.
Dependencies are inferred from the claim text.