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OwnerFrederic J. de Sauvage, Robert D. Klein +2inventors
InventorsFrederic J. de Sauvage, Robert D. Klein, Heidi S. Phillips, Arnon Rosenthal
Estimating the term…
The present invention relates to nucleotide sequences, including expressed sequence tags (ESTs), oligonucleotide probes, polypeptides, vectors and host cells expressing, and immunoadhesions and antibodies to mammalian GFRα3, a novel α-subunit receptor of the GDNF (i.e. GFR) receptor family. It further relates to an assay for measuring activation of an α-subunit receptor by detecting tyrosine kinase receptor activation (i.e., autophosphorylation) or other activities related to ligand-induced α-subunit receptor homo-dimerization or homo-oligomerization.
-25. (canceled)
A method for measuring agonist binding to a polypeptide comprising an agonist-binding domain of an α-subunit receptor, comprising the steps of exposing the polypeptide positioned in a cell membrane to a candidate agonist and measuring homo-dimerization or homo-oligomerization of the polypeptide.
A method of measuring autophosphorylation of a polypeptide receptor construct comprising a ligand-binding domain of an α-subunit receptor, the intracellular catalytic domain of a tyrosine kinase receptor, and a flag epitope, comprising the steps of:
31-40. (canceled)
(canceled)
A method for measuring autophosphorylation of a polypeptide receptor construct comprising a ligand-binding domain of an α-subunit receptor, the intracellular catalytic domain of a tyrosine kinase receptor, and a flag epitope, comprising the steps of:
A polypeptide comprising a GFRα receptor ligand-binding domain, a flag polypeptide, and an intracellular catalytic domain of a tyrosine kinase receptor.
50-66. (canceled)
An assay for measuring phosphorylation of polypeptide receptor construct comprising a ligand-binding domain of an α-subunit receptor, the intracellular catalytic domain of a kinase receptor, and a flag epitope comprising the steps of:
A kit comprising a solid phase coated with a capture agent which binds-specifically to a flag polypeptide, and a polypeptide comprising an α-subunit receptor ligand-binding domain, a flag polypeptide, and an intracellular catalytic domain of a tyrosine kinase receptor.
A method of treating a subject in need of treatment for a condition selected from pain, inflammation, dysfunction of the peripheral nervous system, and dysfunction of the autonomic nervous system, comprising administering to said subject an effective amount of a nucleic acid molecule encoding a GFRα3 receptor comprising the polypeptide of SEQ ID NO:
The method of claim 71, wherein said condition selected from pain, inflammation, dysfunction of the peripheral nervous system, and dysfunction of the autonomic nervous system, comprises a condition selected from neuropathic pain, non-neuropathic pain, chronic pain, chronic back pain, peripheral neuropathy, a disturbance in blood pressure, a disturbance in cardiac rhythm, a gastrointestinal dysfunction, impotence, urinary incontinence, post-herpetic neuralgia, shingles, asthma, irritable bowel, inflammatory bowel, cystitis, headache, migraine, arthritis, spinal cord injury, constipation, hypertension, mucositis, dry mouth, dry eyes, fibromyalgia, and wound healing.
Dependencies are inferred from the claim text.