US 2010/0248238 A1 · Detection Of Bacteria Belonging to the Genus Campylobacter By Targeting Cytolethal Distending Toxin

Abstract

Multiplex PCR primers that can amplify the cdt genes of C. jejuni, C. coli, and C. fetus in a bacterial species-specific manner were prepared. Multiplex PCR with the primers was assessed using Campylobacter bacteria, other cdt gene-positive bacteria, and representative bacteria responsible for enteric infection. As a result, the present inventors' multiplex PCR using cdtB amplification primers was proven to enable simultaneous detection of different Campylobacter bacteria with high specificity. The methods of the present invention can identify Campylobacter at the bacterial species level in a single manipulation even when domestic animals or humans are infected with different bacterial species of Campylobacter.

Claims19

Dependent claims

A method for detecting a Campylobacter bacterium in a test sample, which comprises the step of nucleic acid amplification reaction in the test sample using one or more of primer pairs that comprise two polynucleotides that can specifically bind to genomic DNA or mRNA of cdtB of the Campylobacter bacterium, wherein the primer pairs are:

(a) a primer pair capable of amplifying a genomic DNA region of cdtB of the Campylobacter bacterium which is amplified with a primer pair comprising the sequences of SEQ ID NOs:
1 and 2, or an mRNA region corresponding to the amplifiable genomic DNA region;
and
(b) a primer pair capable of amplifying a genomic DNA region of cdtB of the Campylobacter bacterium which is amplified with a primer pair comprising the sequences of SEQ ID NOs:
3 and 4, or an mRNA region corresponding to the amplifiable genomic DNA region.

2 more elements

A method for detecting a Campylobacter bacterium in a test sample, which comprises the step of nucleic acid amplification reaction in the test sample using a primer pair comprising two polynucleotides that can specifically bind to genomic DNA or mRNA of cdtA of a Campylobacter bacterium, wherein the primer pair is:

(a) a primer pair capable of amplifying a genomic DNA region of cdtA of the Campylobacter bacterium which is amplified with a primer pair comprising the sequences of SEQ ID NOs:
19 and 20, or an mRNA region corresponding to the amplifiable genomic DNA region.

A method for detecting a Campylobacter bacterium in a test sample, which comprises the step of nucleic acid amplification reaction in the test sample using a primer pair comprising two polynucleotides that can specifically bind to genomic DNA or mRNA of cdtC of a Campylobacter bacterium, wherein the primer pair is:

(a) a primer pair capable of amplifying a genomic DNA region of cdtC of the Campylobacter bacterium which is amplified with a primer pair comprising the sequences of SEQ ID NOs:
25 and 26, or an mRNA region corresponding to the amplifiable genomic DNA region.

A method for detecting a Campylobacter bacterium in a test sample, which comprises the step of nucleic acid amplification reaction in a test sample using one or more of:

(a) a primer pair capable of amplifying a genomic DNA region of cdtC of a Campylobacter bacterium that is amplified by a primer pair comprising the sequences of SEQ ID NOs:
37 and 38, or an mRNA region corresponding to the amplifiable genomic DNA region;
(b) a primer pair capable of amplifying a genomic DNA region of cdtC of a Campylobacter bacterium that is amplified by a primer pair comprising the sequences of SEQ ID NOs:
40 and 41, or an mRNA region corresponding to the amplifiable genomic DNA region;
and
(c) a primer pair capable of amplifying a genomic DNA region of cdtC of a Campylobacter bacterium that is amplified by a primer pair comprising the sequences of SEQ ID NOs:
43 and 44, or an mRNA region corresponding to the amplifiable genomic DNA region;
each of which comprises two polynucleotides that can specifically bind to a genomic DNA or mRNA of cdt of the Campylobacter bacterium.

5 more elements

Dependencies are inferred from the claim text.

Detection Of Bacteria Belonging to the Genus Campylobacter By Targeting Cytolethal Distending Toxin

Life of this patent

Detection Of Bacteria Belonging to the Genus Campylobacter By Targeting Cytolethal Distending Toxin

Life of this patent

Abstract

Claims19