US 2010/0248225 A1 · GENE EXPRESSION PROFILING FOR IDENTIFICATION, MONITORING AND TREATMENT OF MELANOMA

Abstract

A method is provided in various embodiments for determining a profile data set for a subject with skin cancer or a condition related to skin cancer based on a sample from the subject, wherein the sample provides a source of RNAs. The method includes using amplification for measuring the amount of RNA corresponding to at least 1 constituent from Tables 1-6. The profile data set comprises the measure of each constituent, and amplification is performed under measurement conditions that are substantially repeatable.

Claims24

Dependent claims

A method for evaluating the presence of melanoma in a subject based on a sample from the subject, the sample providing a source of RNAs, comprising:

a) determining a quantitative measure of the amount of at least one constituent of any constituent of any one table selected from the group consisting of Tables 1, 2, 3, 4, 5 and 6 as a distinct RNA constituent in the subject sample, wherein such measure is obtained under measurement conditions that are substantially repeatable and the constituent is selected so that measurement of the constituent distinguishes between a normal subject and a melanoma-diagnosed subject in a reference population with at least 75% accuracy;
and
b) comparing the quantitative measure of the constituent in the subject sample to a reference value.

A method for assessing or monitoring the response to therapy in a subject having melanoma based on a sample from the subject, the sample providing a source of RNAs, comprising:

a) determining a quantitative measure of the amount of at least one constituent of any constituent of Tables 1, 2, 3, 4, 5, and 6 as a distinct RNA constituent, wherein such measure is obtained under measurement conditions that are substantially repeatable to produce subject data set;
and
b) comparing the subject data set to a baseline data set.

A method for monitoring the progression of melanoma in a subject, based on a sample from the subject, the sample providing a source of RNAs, comprising:

a) determining a quantitative measure of the amount of at least one constituent of any constituent of Tables 1, 2, 3, 4, 5, and 6 as a distinct RNA constituent in a sample obtained at a first period of time, wherein such measure is obtained under measurement conditions that are substantially repeatable to produce a first subject data set;
b) determining a quantitative measure of the amount of at least one constituent of any constituent of Tables 1, 2, 3, 4, 5, and 6 as a distinct RNA constituent in a sample obtained at a second period of time, wherein such measure is obtained under measurement conditions that are substantially repeatable to produce a second subject data set;
and
c) comparing the first subject data set and the second subject data set.

1 more element

A method for determining a melanoma profile based on a sample from a subject known to have melanoma, the sample providing a source of RNAs, the method comprising:

a) using amplification for measuring the amount of RNA in a panel of constituents including at least 1 constituent from Tables 1, 2, 3, 4, 5, and 6 and
b) arriving at a measure of each constituent,
wherein the profile data set comprises the measure of each constituent of the panel and wherein amplification is performed under measurement conditions that are substantially repeatable.

Dependencies are inferred from the claim text.

Facts

Application

12/312,390Filed 6 Nov 2007

Priority

6 Nov 2006

Published

30 Sep 2010

Term ends

Not estimatedThe source records don’t support an estimate.

Classification5 codes

C12Q 1/6886
For diseases caused by alterations of genetic material: for cancer
C12Q 2600/118
Oligonucleotides characterized by their use: prognosis of disease development
C12Q 2600/136
Oligonucleotides characterized by their use: screening for pharmacological compounds
C12Q 2600/158
Oligonucleotides characterized by their use: expression markers

Gene expression profiling for identification, monitoring and treatment of melanoma

Life of this patent

Gene expression profiling for identification, monitoring and treatment of melanoma

Life of this patent

Abstract

Claims24