US 2010/0248220 A1 · Chlamydia Trachomatis Specific Oligonucleotide Sequences

Abstract

The present invention relates to oligonucleotide sequences for amplification primers and detection probes and to their use in nucleic acid amplification methods for the selective and specific detection of Chlamydia trachomatis in biological samples. The invention also provides oligonucleotide primer sets and primer/probe sets in the form of kits for the detection and diagnosis of chlamydial infection. The inventive oligonucleotide primers and probes can also be used in combination with other specific oligonucleotide primers and probes for the simultaneous detection of Chlamydia trachomatis and other target organisms, such as Neisseria gonorrhea.

Claims39

Dependent claims

An isolated oligonucleotide comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs. 1-41, active fragments thereof, and combinations thereof.

A collection of oligonucleotides for detecting Chlamydia trachomatis in a test sample comprising primer sets selected from the group consisting of:

Primer Set 1, Primer Set 2, Primer Set 3, Primer Set 4, Primer Set 5, Primer Set 6, Primer Set 7, Primer Set 8, Primer Set 9, Primer Set 10, Primer Set 11, and Primer Set CT(mpx), wherein:
Primer Set 1 comprises a forward primer comprising SEQ. ID NO. 1 or any active fragment thereof, and a reverse primer comprising SEQ. ID NO. 2 or any active fragment thereof;
Primer Set 2 comprises a forward primer comprising SEQ. ID NO. 5 or any active fragment thereof, and a reverse primer comprising SEQ. ID NO. 6 or any active fragment thereof;
Primer Set 3 comprises a forward primer comprising SEQ. ID NO. 9 or any active fragment thereof, and a reverse primer comprising SEQ. ID NO. 10 or any active fragment thereof;
Primer Set 4 comprises a forward primer comprising SEQ. ID NO. 13 or any active fragment thereof, and a reverse primer comprising SEQ. ID NO. 14 or any active fragment thereof;
Primer Set 5 comprises a forward primer comprising SEQ. ID NO. 16 or any active fragment thereof, and a reverse primer comprising SEQ. ID NO. 17 or any active fragment thereof,
Primer Set 6 comprises a forward primer comprising SEQ. ID NO. 20 or any active fragment thereof, and a reverse primer comprising SEQ. ID NO. 21 or any active fragment thereof;
Primer Set 7 comprises a forward primer comprising SEQ. ID NO. 23 or any active fragment thereof, and a reverse primer comprising SEQ. ID NO. 24 or any active fragment thereof;
Primer Set 8 comprises a forward primer comprising SEQ. ID NO. 26 or any active fragment thereof, and a reverse primer comprising SEQ. ID NO. 27 or any active fragment thereof;
Primer Set 9 comprises a forward primer comprising SEQ. ID NO. 29 or any active fragment thereof, and a reverse primer comprising SEQ. ID NO. 30 or any active fragment thereof;
Primer Set 10 comprises a forward primer comprising SEQ. ID NO. 32 or any active fragment thereof, and a reverse primer comprising SEQ. ID NO. 33 or any active fragment thereof;
Primer Set 11 comprises a forward primer comprising SEQ. ID NO. 35 or any active fragment thereof, and a reverse primer comprising SEQ. ID NO. 36 or any active fragment thereof;
and
Primer Set CT(mpx) comprises a first forward primer comprising SEQ. ID NO. 38 or any active fragment thereof, a second forward primer comprising SEQ. ID NO. 39 or any active fragment thereof, and a reverse primer comprising SEQ. ID NO. 40 or any active fragment thereof.

11 more elements

A collection of oligonucleotides for detecting Chlamydia trachomatis in a test sample comprising primer/probe sets selected from the group consisting of:

Primer/Probe set CT1, Primer/Probe set CT2-P1, Primer/Probe set CT2-P2, Primer/Probe set CT3, Primer/Probe set CT4, Primer/Probe set CT5, Primer/Probe set CT6, Primer/Probe set CT7, Primer/Probe set CT8, Primer/Probe set CT9, Primer/Probe set CT10, Primer/Probe set CT11, and Primer/Probe Set CT(mpx), wherein:
Primer/Probe Set CT1 comprises a forward primer comprising SEQ. ID NO. 1 or an active fragment thereof, a reverse primer comprising SEQ. ID NO. 2 or an active fragment thereof, a complementary detection probe comprising SEQ. ID NO. 3 or an active fragment thereof, and a reverse complementary detection probe comprising SEQ. ID NO. 4 or an active fragment thereof;
Primer/Probe Set CT2-P1 comprises a forward primer comprising SEQ. ID NO. 5 or an active fragment thereof, a reverse primer comprising SEQ. ID NO. 6 or an active fragment thereof, and a complementary detection probe comprising SEQ. ID NO. 7 or an active fragment thereof;
Primer/Probe Set CT2-P2 comprises a forward primer comprising SEQ. ID NO. 5 or an active fragment thereof, a reverse primer comprising SEQ. ID NO. 6 or an active fragment thereof, and a complementary detection probe comprising SEQ. ID NO. 8 or an active fragment thereof;
Primer/Probe Set CT3 comprises a forward primer comprising SEQ. ID NO. 9 or an active fragment thereof, a reverse primer comprising SEQ. ID NO. 10 or an active fragment thereof, a complementary detection probe comprising SEQ. ID NO. 11 or an active fragment thereof and a reverse complementary detection probe comprising SEQ. ID NO. 12 or an active fragment thereof;
Primer/Probe Set CT4 comprises a forward primer comprising SEQ. ID NO. 13 or an active fragment thereof, a reverse primer comprising SEQ. ID NO. 14 or an active fragment thereof, and a complementary detection probe comprising SEQ. ID NO. 15 or an active fragment thereof;
Primer/Probe Set CT5 comprises a forward primer comprising SEQ. ID NO. 16 or an active fragment thereof, a reverse primer comprising SEQ. ID NO. 17 or an active fragment thereof, a complementary detection probe comprising SEQ. ID NO. 18 or an active fragment thereof, and a reverse complementary detection probe comprising SEQ. ID NO. 19 or an active fragment thereof;
Primer/Probe Set CT6 comprises a forward primer comprising SEQ. ID NO. 20 or an active fragment thereof, a reverse primer comprising SEQ. ID NO. 21 or an active fragment thereof, a complementary detection probe comprising SEQ. ID NO. 22 or an active fragment thereof;
Primer/Probe Set CT7 comprises a forward primer comprising SEQ. ID NO. 23 or an active fragment thereof, a reverse primer comprising SEQ. ID NO. 24 or an active fragment thereof, and a complementary detection probe comprising SEQ. ID NO. 25 or an active fragment thereof;
Primer/Probe Set CT8 comprises a forward primer comprising SEQ. ID NO. 26 or an active fragment thereof, a reverse primer comprising SEQ. ID NO. 27 or an active fragment thereof, and a complementary detection probe comprising SEQ. ID NO. 28 or an active fragment thereof;
Primer/Probe Set CT9 comprises a forward primer comprising SEQ. ID NO. 29 or an active fragment thereof, a reverse primer comprising SEQ. ID NO. 30 or an active fragment thereof, and a complementary detection probe comprising SEQ. ID NO. 31 or an active fragment thereof;
Primer/Probe Set CT10 comprises a forward primer comprising SEQ. ID NO. 32 or an active fragment thereof, a reverse primer comprising SEQ. ID NO. 33 or an active fragment thereof, and a complementary detection probe comprising SEQ. ID NO. 34 or an active fragment thereof;
Primer/Probe Set CT11 comprises a forward primer comprising SEQ. ID NO. 35 or an active fragment thereof, a reverse primer comprising SEQ. ID NO. 36 or an active fragment thereof, and a complementary detection probe comprising SEQ. ID No. 37 or an active fragment thereof;
and
Primer/Probe Set CT(mpx) comprises a first forward amplification primer comprising SEQ. ID NO. 38 or an active fragment thereof, a second forward amplification primer comprising SEQ. ID NO. 39 or an active fragment thereof, a reverse amplification primer comprising SEQ. ID NO. 40 or an active fragment thereof, and a detection probe comprising SEQ. ID NO. 41 or an active fragment thereof.

12 more elements

Dependencies are inferred from the claim text.

Chlamydia Trachomatis Specific Oligonucleotide Sequences

Life of this patent

Chlamydia Trachomatis Specific Oligonucleotide Sequences

Life of this patent

Abstract

Claims39